Compositions and methods for rapid and dynamic flux control using synthetic metabolic valves

A genetically modified microorganism comprising:

• a production pathway comprising at least one enzyme for producing of an isoprenoid product, and
• at least one synthetic metabolic valve characterized by both (i) silencing of gene expression of a gene encoding a first enzyme essential for growth of the genetically modified microorganism and (ii) proteolysis of a second enzyme essential for growth of the genetically modified microorganism,
• wherein under condition of depleting of a limiting nutrient from a growth media in which the genetically modified microorganism is growing will inducing a stationary or non-dividing cellular state;
• and
• wherein the synthetic metabolic valve of the microorganism is conditionally triggered;
• wherein the gene encoding the first enzyme essential for growth of the genetically modified microorganism is one of:
• enoyl-ACP reductase (fabI), citrate synthase (gltA), soluble transhydrogenase (udhA), glucose-6-phosphate-1-dehydrogenase (zwf), or lipoamide dehydrogenase (lpd), and combinations thereof;
• and
• wherein the second enzyme is one of:
• enoyl-ACP reductase (fabI), citrate synthase (gltA), soluble transhydrogenase (udhA), glucose-6-phosphate-1-dehydrogenase (zwf), or lipoamide dehydrogenase (lpd), and combinations thereof.