Method for the preparation of nanoscale DNA-encircled lipid bilayers
AssigneesTechnische UniversitäT Dresden, Helmholtz-Zentrum Dresden-Rossendorf E.V.
InventorsKarim Fahmy, Thorsten-Lars Schmidt, Katarina Iric
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Abstract
The present invention relates to a method for the preparation of nanoscale nucleic acid-encircled lipid bilayers, the nanoscale nucleic acid-encircled lipid bilayers and their use.
Claims20
A minicircle-surrounded lipid bilayer at least comprising one membrane associated entity, wherein the minicircle comprises at least nucleic acids or nucleic acid analogues, and wherein the membrane associated entity is selected from peptides and proteins which are associated to biological membranes.
A method for preparation of minicircle-surrounded lipid bilayers comprising the steps of:
- a) providing a minicircle comprising at least double stranded nucleic acids or nucleic acid analogues,
- b) mixing of the minicircle with lipids and a membrane associated entity in a detergent, wherein the membrane associated entity is selected from peptides and proteins which are associated to biological membranes,
- c) removing the detergent, and
- d) self-assembly of the minicircle-surrounded lipid bilayer comprising the membrane associated entity.
Dependencies are inferred from the claim text.