US 11,098,287 B2 · 17β-hydroxysteroid dehydrogenase mutants and application thereof

Abstract

The disclosure discloses 17β-hydroxysteroid dehydrogenase mutants and application thereof, and belongs to the technical field of biology. The disclosure provides 17β-hydroxysteroid dehydrogenase mutants V107A, T155N, H164Y and V107A/T155N/H164Y with high specific enzyme activities, and the specific enzyme activities of the 17β-hydroxysteroid dehydrogenase mutants V107A, T155N, H164Y and V107A/T155N/H164Y are as high as 1.85, 1.93, 2.06 and 5.15 U/mg, respectively, which are 1.11, 1.16, 1.24 and 3.10 times larger than that of wild-type 17β-hydroxysteroid dehydrogenase (1.66 U/mg).

Claims9

Dependent claims

A 17β-hydroxysteroid dehydrogenase mutant, wherein the 17β-hydroxysteroid dehydrogenase mutant comprises an amino acid sequence having all of SEQ ID NO:

2 except for:
a mutation of valine at position 107 to alanine;
a mutation of threonine at position 155 to asparagine;
a mutation of histidine at position 164 to tyrosine;
or
a mutation of threonine at position 155 to asparagine, and a mutation of histidine at position 164 to tyrosine.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

17/094,982Filed 11 Nov 2020

Priority

CN 202010945382.010 Sep 2020

Published

US 2021/0087539 A125 Mar 2021

Granted

24 Aug 2021

Term ends

Not estimatedThe source records don’t support an estimate.

Classification10 codes

C12N 9/0006
Oxidoreductases: acting on CH-OH groups as donors
C12N 1/20
Microorganisms; Compositions thereof ; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of…:…
C12N 15/52
DNA or RNA fragments; Modified forms thereof ; Non-coding nucleic acids having a biological activity: genes encoding for enzymes or proenzymes
C12N 15/70
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression: vectors or expression systems…

17β-hydroxysteroid dehydrogenase mutants and application thereof

Life of this patent

17β-hydroxysteroid dehydrogenase mutants and application thereof

Life of this patent

Abstract

Claims9