Coiled-coil connector

A multimeric protein comprising a first polypeptide and a second polypeptide joined by a connector, the connector consisting of a coiled coil-type structure consisting of a first peptidic alpha-helix and a second peptidic alpha-helix, wherein the first and the second peptidic alpha-helices form the coiled coil-type structure,

• wherein:
• i) the first peptidic alpha-helix consists of repeats of the amino acid motif a-x1 and a C-terminal motif a-x1-x2, and the second peptidic alpha helix consists of repeats of an amino acid motif x1-a selected from the group consisting of SEQ ID NOs:
• 2, 4, 6, and 8, wherein the first polypeptide of the multimeric protein is fused to the N-terminus of the first peptidic alpha-helix, and the second polypeptide of the multimeric protein is fused to the N-terminus or to the C-terminus of the second peptidic alpha-helix;
• or
• ii) the first peptidic alpha-helix consists of an N-terminal motif x2-x1-a and repeats of the amino acid motif x1-a, and the second alpha-helix consists of repeats of an amino acid motif a-x1 selected from the group consisting of SEQ ID NOs:
• 1, 3, 5, and 7, wherein the first polypeptide of the multimeric protein is fused to the C-terminus of the first peptidic alpha-helix, and the second polypeptide of the multimeric protein is fused to the N-terminus or to the C-terminus of the second peptidic alpha-helix;
• wherein in the first peptidic alpha-helix:
• a1) the repeating motif a-x1 is SEQ ID NO:1 and the C-terminal motif a-x1-x2 is SEQ ID NO:1 with a C-terminal extension x2 consisting of 1-2 amino acids;
• a2) the repeating motif a-x1 is SEQ ID NO:1 and the C-terminal motif a-x1-x2 is SEQ ID NO:41, SEQ ID NO:69, or SEQ ID NO:71;
• b1) the repeating motif a-x1 is SEQ ID NO:3 and the C-terminal motif a-x1-x2 is SEQ ID NO:3 with a C-terminal extension x2 consisting of 1-2 amino acids;
• b2) the repeating motif a-x1 is SEQ ID NO:3 and the C-terminal motif a-x1-x2 is SEQ ID NO:43, SEQ ID NO:85, or SEQ ID NO:87;
• c1) the repeating motif a-x1 is SEQ ID NO:5 and the C-terminal motif a-x1-x2 is SEQ ID NO:5 with a C-terminal extension x2 consisting of 1-2 amino acids;
• c2) the repeating motif a-x1 is SEQ ID NO:5 and the C-terminal motif a-x1-x2 is SEQ ID NO:45, SEQ ID NO:101, or SEQ ID NO:103;
• d1) the repeating motif a-x1 is SEQ ID NO:7 and the C-terminal motif a-x1-x2 is SEQ ID NO:7 with a C-terminal extension x2 consisting of 1-2 amino acids;
• d2) the repeating motif a-x1 is SEQ ID NO:7 and the C-terminal motif a-x1-x2 is SEQ ID NO:47, SEQ ID NO:117, or SEQ ID NO:119;
• e1) the repeating motif x1-a is SEQ ID NO:2 and the N-terminal motif x2-x1-a is SEQ ID NO:2 with an N-terminal extension x2 consisting of 1-2 amino acids;
• e2) the repeating motif x1-a is SEQ ID NO:2 and the N-terminal motif x2-x1-a is SEQ ID NO:42, SEQ ID NO:77, or SEQ ID NO:79;
• f1) the repeating motif x1-a is SEQ ID NO:4 and the N-terminal motif x2-x1-a is SEQ ID NO:4 with an N-terminal extension x2 consisting of 1-2 amino acids;
• f2) the repeating motif x1-a is SEQ ID NO:4 and the N-terminal motif x2-x1-a is SEQ ID NO:44, SEQ ID NO:93, or SEQ ID NO:95;
• g1) the repeating motif x1-a is SEQ ID NO:6 and the N-terminal motif x2-x1-a is SEQ ID NO:6 with an N-terminal extension x2 consisting of 1-2 amino acids;
• g2) the repeating motif x1-a is SEQ ID NO:6 and the N-terminal motif x2-x1-a is SEQ ID NO:46, SEQ ID NO:109, or SEQ ID NO:111;
• h1) the repeating motif x1-a is SEQ ID NO:8 and the N-terminal motif x2-x1-a is SEQ ID NO:8 with an N-terminal extension x2 consisting of 1-2 amino acids;
• or
• h2) the repeating motif x1-a is SEQ ID NO:8 and the N-terminal motif x2-x1-a is SEQ ID NO:48, SEQ ID NO:125, or SEQ ID NO:127.