US 11,085,927 B2 · Techniques for high throughput targeted proteomic analysis and related systems and methods

Abstract

Described herein is a kit of materials prepared for assays that involve determining relative abundance and/or absolute abundance of various targeted peptides. The kit may comprise trigger versions of target peptides with masses offset from the respective target peptides by predetermined and known amounts. The trigger peptides may be present in amounts that may be readily detected via a mass spectrometry analysis. When mixed with samples that are analyzed, detection of the trigger peptides indicates where in the mass-spectrometer output the target peptide may be found. The kit may include a predetermined amount of synthetic versions of one or more of the target peptides. A measured relative abundance of this synthetic peptide relative to that of the target peptides yields an absolute quantitative value of the target peptide. Also disclosed is a method of preparing a plurality of samples to be submitted for mass spectrometer analysis in parallel.

Claims18

Dependent claims

A kit for performing an assay to determine a disease and/or condition state of an organism, the kit comprising:

a plurality of peptides proteotypic of a protein whose expression is characteristic of the disease and/or condition state, wherein the plurality of peptides are mass-tagged peptides,
wherein the plurality of peptides includes a population of a first peptide mass-tagged with a first mass tag, the population of the first peptide comprising:
a first plurality of molecules of the first peptide mass-tagged with the first mass tag, each molecule of the first plurality of molecules having a first mass, and
a second plurality of molecules of the first peptide mass-tagged with the first mass tag, each molecule of the second plurality of molecules having a second mass, different from the first mass,
wherein molecules of the second plurality of molecules are chemically identical to one another and to each molecule of the first plurality of molecules;
and
information indicating:
the first mass as a mass spectrometry trigger;
the first mass tag;
and
the second mass.

8 more elements

Dependencies are inferred from the claim text.

Techniques for high throughput targeted proteomic analysis and related systems and methods

Life of this patent

Techniques for high throughput targeted proteomic analysis and related systems and methods

Life of this patent

Abstract

Claims18