Ungulates with genetically modified immune systems
AssigneeRevivicor, Inc.
InventorsKevin Wells, David Ayares
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Abstract
The present invention provides ungulate animals, tissue and organs as well as cells and cell lines derived from such animals, tissue and organs, which lack expression of functional endogenous immunoglobulin loci. The present invention also provides ungulate animals, tissue and organs as well as cells and cell lines derived from such animals, tissue and organs, which express xenogenous, such as human, immunoglobulin loci. The present invention further provides ungulate, such as porcine genomic DNA sequence of porcine heavy and light chain immunogobulins. Such animals, tissues, organs and cells can be used in research and medical therapy. In addition, methods are provided to prepare such animals, organs, tissues, and cells.
Claims15
A targeting vector that inactivates a porcine lambda light chain genetic locus, wherein the target vector comprises:
- (a) a first nucleotide sequence comprising at least 30 contiguous nucleic acids of a nucleotide sequence that flanks the 5′ end of the first joining/constant (J/C) region of the porcine lambda light chain genetic locus;
- (b) a selectable marker gene;
- and
- (c) a second-nucleotide sequence comprising at least 30 contiguous nucleic acids of a nucleotide sequence that flanks the 3′ end of the J/C cluster region of the porcine lambda light chain genetic locus.
Dependencies are inferred from the claim text.