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US 11,085,036 B2Granted
Modulating polymer beads for DNA processing
AssigneeIllumina, Inc.
InventorsSteven Norberg, Dmitry Pokholok, Ramesh Ramji, Frank J. Steemers, Fan Zhang
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Abstract
Systems, methods, and compositions provided herein relate to preparation of beads encapsulating biomolecules for performing sequential reactions on the biomolecules. Some embodiments include preparation of nucleic acid reactions within the bead, wherein the bead includes pores that allow diffusion of molecules into or out of the beads while retaining other molecules of interest.
Claims29
A polymer bead for performing multiple co-assay reactions, comprising:
- a hydrogel polymer precursor;
- a crosslinker;
- and
- a biomolecule disposed within the polymer bead, wherein the bead is a porous hollow bead that comprises multiple polymer layers, wherein each polymer layer comprises a distinct pore size and a distinct pore density that allows diffusion of a reagent through the bead while retaining the biomolecule.
1 more element
Dependencies are inferred from the claim text.