Glycosylated oxalate decarboxylase and preparation and application thereof

A glycosylated oxalate decarboxylase, wherein:

• the oxalate decarboxylase is derived from edible fungi of Agrocybe, the glycosylated oxalate decarboxylase has an enzyme activity at the pH value of 1.5-7.5;
• the optimal pH value of the enzyme activity of the oxalate decarboxylase is pH 2.5-3.0;
• when the oxalate decarboxylase is at the optimal pH value, the optimal activity is 20-200 U/mg;
• the activity at the pH value of 1.5-2.0 is greater than 10% of the optimum activity and the specific activity is more than 2 U/mg;
• the activity at the pH value of 2.0-5.5 is more than 30% of the optimal activity, and the specific activity is more than 6 U/mg;
• and
• the oxalate decarboxylase is inductively produced by the following liquid fermentation method:
• after 2-5 days of culture in a fermentation broth of the edible fungi, an acid is added to adjust the pH value to pH 2.0-4.0 under the condition of 0.1-6 mM manganese ions, and then the enzyme is inductively produced;
• and continuously cultured 7-10 days.