Method for producing a structure with spatial encoded functionality

Method for producing a structure with spatial encoded functionality, the method comprising:

• providing, in a volume formed on a glass substrate, a first photosensitive material that is two-photon crosslinking compatible, wherein the first photosensitive material is a hydrogel,
• generating in the volume a framework of crosslinked first photosensitive material, the generating of the framework comprising exposing the first photosensitive material with a first focused laser beam according to a first pattern for specifically initiating a two-photon crosslinking of the first photosensitive material in accordance with the first pattern,
• removing from the volume any remaining non-crosslinked portions of the first photosensitive material, wherein the framework comprises an array of first structures,
• providing to the volume a second photosensitive material that is two-photon crosslinking compatible,
• generating in the volume the structure, the generating of the structure comprising exposing the second photosensitive material with a second focused laser beam according to a second pattern for specifically initiating a two-photon crosslinking of predefined surface portions of each first structure of the array of first structures and the second photosensitive material in accordance with the second pattern,
• removing from the volume any remaining non-crosslinked portions of the second photosensitive material, the remaining crosslinked portions of the second photosensitive material having reactive sites, wherein the structure comprises the array of first structures having respective reactive sites, wherein each first structure of the array of first structures is separated from remaining first structures of the array of first structures, wherein each first structure of the array of first structures is a microswimmer, and wherein each microswimmer has a pointy head chemically bonded to the glass substrate, and
• chemically modifying at least part of reactive sites with one or more chemicals until the reactive sites possess a desired encoded functionality, wherein the chemicals are chemically inert to the first photosensitive material.