Biosensors for biological or chemical analysis and systems and methods for same

A biosensor comprising:

• a detection device comprising a plurality of stacked layers and having a detector surface configured to support reaction sites, the stacked layers including a filter layer and a solid-state imager coupled to the filter layer, the filter layer comprising filter walls and a light-absorbing material that is deposited between adjacent filter walls, the light-absorbing material configured to prevent transmission of excitation light and permit transmission of emission signals, wherein the adjacent filter walls define a detection path therebetween through the corresponding light-absorbing material toward the solid-state imager, the filter walls configured to reflect the emission signals;
• a coating covering at least a portion of the filter walls, the coating interposed between the filter wall and the light-absorbing material;
• and
• a passivation layer positioned above the filter layer.