Method for measurement of peptidic degradation products of a proteolytic cascade in blood samples
AssigneeAttoquant Diagnostics GmbH
InventorsMarko Poglitsch, Cornelia Schwager, Hans Loibner, Manfred Schuster
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Abstract
The invention discloses a method for measurement of peptidic degradation products of a proteolytic cascade in biological samples, especially blood samples, wherein the sample is incubated until a steady state equilibrium is reached for at least one peptidic degradation product involved in said proteolytic cascade and wherein said at least one peptidic degradation product in steady state equilibrium of the proteolytic cascade is quantified in the sample.
Claims27
A method for measurement of peptidic degradation products of a renin-angiotensin system and/or bradykinin proteolytic cascade in a biological sample comprising:
- incubating a biological sample ex vivo at a temperature of 30 to 50° C. until a steady state equilibrium based on concentrations is reached for at least two peptidic degradation products involved in a renin-angiotensin system and/or bradykinin proteolytic cascade;
- measuring concentrations of the at least two peptidic degradation products in steady state equilibrium in the sample;
- and
- calculating a ratio of the measured steady state equilibrium concentrations of the at least two peptidic degradation products,
- wherein substrates or substrate analogues of any enzyme(s) involved in the proteolytic cascade are not added before and/or during the incubation until a steady state equilibrium is reached.
A method for measurement of peptidic degradation products of a renin-angiotensin system and/or bradykinin proteolytic cascade in a biological sample comprising:
- incubating a biological sample ex vivo at a temperature of 30 to 50° C. until a steady state equilibrium based on concentrations is reached for at least two peptidic degradation products involved in a renin-angiotensin system and/or bradykinin proteolytic cascade;
- measuring concentrations of the at least two peptidic degradation products in steady state equilibrium in the sample;
- and
- calculating a ratio of the measured steady state equilibrium concentrations of the at least two peptidic degradation products,
- wherein substrates or substrate analogues of any enzyme(s) involved in the proteolytic cascade are not added before and/or during the incubation until a steady state equilibrium is reached,
- and wherein the peptidic degradation products measured comprise angiotensin II, angiotensin 1-7, and angiotensin 1-5.
Dependencies are inferred from the claim text.