# US 11,079,376 B1 · Optical analyte detector

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- Number: US 11,079,376 B1
- Document ID: US11079376B1
- Status: Granted
- Kind: B1
- Application number: 17/096,747
- Filed: 2020-11-12
- Granted: 2021-08-03
- Earliest claimed: 2020-11-12
- Assignees: United Arab Emirates University
- Applicants: United Arab Emirates University
- Inventors: Adel Najar
- Web page: https://patentgrep.com/patent/US11079376B1

## CPC

- G01N 21/77: by observing the effect on a chemical indicator
- G01N 33/54373: {involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings}
- G01N 21/25: Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N 33/54366: {Apparatus specially adapted for solid-phase testing}
- G01N 33/56983: {Viruses}
- G02B 6/12004: {Combinations of two or more optical elements}
- G02B 6/12007: {forming wavelength selective elements, e.g. multiplexer, demultiplexer}
- G01N 2021/7789: {Cavity or resonator}
- G01N 2201/06113: Coherent sources; lasers
- G01N 2201/08: Optical fibres; light guides
- G01N 2333/165: Coronaviridae, e.g. avian infectious bronchitis virus
- G01N 2469/10: Detection of antigens from microorganism in sample from host
- G02B 6/29338: {Loop resonators}
- G02B 6/4215: {the intermediate optical elements being wavelength selective optical elements, e.g. variable wavelength optical modules or wavelength lockers (G02B6/4246 takes precedence)}
- G02B 2006/12038: {Glass (SiO2 based materials)}
- G02B 2006/12121: {Laser}
- G02B 2006/12123: {Diode}
- G02B 2006/12138: {Sensor}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- G02B: OPTICAL ELEMENTS, SYSTEMS OR APPARATUS

## Abstract

The optical analyte detector is a photonic detector that uses a measured wavelength shift to determine the presence of an analyte. An open cell is formed in an optical layer for receiving a sample to be analyzed. A transition metal dichalcogenide monolayer defines a bottom wall of the open cell, and the transition metal dichalcogenide monolayer is formed directly above a microring resonator. A waveguide is positioned adjacent to the open cell, and is spaced apart therefrom by a gap. The waveguide is coupled to the microring resonator, and the transition metal dichalcogenide monolayer is functionalized with an adsorbed layer for detection of a specific analyte. Molecular binding takes place if a sample of the analyte contacts the adsorbed layer, which induces a wavelength shift in light transmitted through the waveguide. The presence of this measured wavelength shift indicates positive detection of the analyte.

## Claims

### Claim 1

An optical analyte detector, comprising:
a light source for generating a first optical signal having a first wavelength;
a substrate;
an optical layer formed on the substrate, the optical layer having an upper surface;
first and second open cells defined in the upper surface of the optical layer, the first open cell being adapted for receiving a reference sample and the second open cell being adapted for receiving a test sample to be analyzed for presence of the analyte;
a first waveguide embedded in the optical layer, the light source transmitting the first optical signal into the first waveguide;
second, third and fourth waveguides embedded in the optical layer, the fourth waveguide directly optically coupling the light source to the first waveguide;
a primary microring resonator embedded in the optical layer adjacent the fourth waveguide, the primary microring resonator being optically coupled to the fourth waveguide;
a first optical splitter disposed between the first waveguide and the second and third waveguides, the first optical splitter splitting the first optical signal between the second and third waveguides into waves of equal power transmission, defining second and third optical signals, respectively;
a first transition metal dichalcogenide monolayer embedded in the optical layer, the first transition metal dichalcogenide monolayer defining a bottom wall of the first open cell;
a second transition metal dichalcogenide monolayer embedded in the optical layer, the second transition metal dichalcogenide monolayer defining a bottom wall of the second open cell;
a first microring resonator embedded in the optical layer beneath the first transition metal dichalcogenide monolayer and coupled thereto, the second waveguide being positioned to optically couple with the first microring resonator;
a second microring resonator embedded in the optical layer beneath the second transition metal dichalcogenide monolayer and coupled thereto, the third waveguide being positioned to optically couple with the second microring resonator;
a first photodetector receiving the second optical signal from the second waveguide, the second waveguide, the first open cell, the first microring resonator, and the first photodetector defining a reference branch;
a second photodetector receiving the third optical signal from the third waveguide, the third waveguide, the second open cell, the second microring resonator, and the second photodetector defining a test branch; and
a first comparator in communication with the first and second photodetectors for comparing wavelengths of the optical signals in the reference branch and the test branch and calculating a first wavelength shift therebetween, the detector confirming presence of the analyte in the test sample when the first wavelength shift is greater than 0.01 nm.

### Claim 2 (depends on 1)

The optical analyte detector as recited in claim 1, wherein the substrate comprises a silicon or silicon carbide layer.

### Claim 3 (depends on 1)

The optical analyte detector as recited in claim 1, wherein the optical layer comprises a silicon dioxide layer.

### Claim 4 (depends on 1)

The optical analyte detector as recited in claim 1, wherein the first, second and third waveguides each comprise silicon or silicon carbide.

### Claim 5 (depends on 1)

The optical analyte detector as recited in claim 1, wherein each of the first and second transition metal dichalcogenide monolayers comprises a transition metal dichalcogenide selected from the group consisting of MoS2, MoSe2, WS2, and WSe2.

### Claim 6 (depends on 1)

The optical analyte detector as recited in claim 1, wherein each of the first and second transition metal dichalcogenide monolayers is functionalized with an adsorbed layer for detection of a particular analyte.

### Claim 7 (depends on 1)

The optical analyte detector as recited in claim 1, further comprising:
third and fourth open cells defined in the upper surface of the optical layer, the third open cell being adapted for receiving a second reference sample and the fourth open cell being adapted for receiving a second test sample to be analyzed for presence of the analyte;
fifth, sixth and seventh waveguides embedded in the optical layer;
a second optical splitter disposed between the fourth waveguide and the first and fifth waveguides, the second optical splitter splitting the first optical signal between the first and fifth waveguides into waves of equal power transmission;
a third optical splitter disposed between the fifth waveguide and the sixth and seventh waveguides, the third optical splitter splitting the first optical signal between the sixth and seventh waveguides into waves of equal power transmission, defining fourth and fifth optical signals, respectively;
a third transition metal dichalcogenide monolayer embedded in the optical layer, the third transition metal dichalcogenide monolayer defining a bottom wall of the third open cell;
a fourth transition metal dichalcogenide monolayer embedded in the optical layer, the fourth transition metal dichalcogenide monolayer defining a bottom wall of the fourth open cell;
a third microring resonator embedded in the optical layer beneath the third transition metal dichalcogenide monolayer and coupled thereto, the sixth waveguide being positioned to optically couple with the third microring resonator;
a fourth microring resonator embedded in the optical layer beneath the fourth transition metal dichalcogenide monolayer and coupled thereto, the seventh waveguide being positioned to optically couple with the fourth microring resonator;
a third photodetector receiving the fourth optical signal from the sixth waveguide, the sixth waveguide, the third open cell, the third microring resonator, and the third photodetector defining a second reference branch;
a fourth photodetector receiving the fifth optical signal from the seventh waveguide, the seventh waveguide, the fourth open cell, the fourth microring resonator, and the fourth photodetector defining a second test branch; and
a second comparator in communication with the third and fourth photodetectors for comparing wavelengths of the optical signals in the second reference branch and the second test branch and calculating a second wavelength shift therebetween, the detector confirming presence of the analyte in the second test sample when the second wavelength shift is substantially non-zero.

### Claim 8 (depends on 7)

The optical analyte detector as recited in claim 7, wherein the fourth, fifth, sixth and seventh waveguides each comprise silicon or silicon carbide.

### Claim 9 (depends on 8)

The optical analyte detector as recited in claim 8, wherein each of the third and fourth transition metal dichalcogenide monolayers comprises a transition metal dichalcogenide selected from the group consisting of MoS2, MoSe2, WS2, and WSe2.

### Claim 10 (depends on 7)

The optical analyte detector as recited in claim 7, wherein each of the third and fourth transition metal dichalcogenide monolayers is functionalized with an adsorbed layer for detection of a particular analyte.

### Claim 11 (depends on 1)

The optical analyte detector according to claim 1, wherein said light source comprises a III-V vertical-cavity surface-emitting laser (VCSEL).

### Claim 12 (depends on 1)

The optical analyte detector according to claim 1, wherein said photodetectors comprise photodiodes.

### Claim 13

A method for detecting COVID-19 infection using a silicon photonic biosensor device, comprising the steps of:
directing an optical signal through a waveguide formed on a silicon photonic biosensor device;
splitting the optical signal with a 1:1 power splitter into a reference optical signal propagating through a reference waveguide formed on the device and a test optical signal propagating through a test waveguide formed on the device;
placing a volume of a reference solution containing at least one antibody selectively binding to SARS-COV-2 virus in a reference cavity formed in a surface of the device above a reference microring resonator optically coupled to the reference waveguide, the device having a monolayer of a transition metal dichalcogenide disposed between the reference cavity and the reference microring resonator;
placing a volume of a test solution containing the antibody selectively binding to SARS CoV-2 virus in a test cavity formed in the surface of the device above a test microring resonator optically coupled to the test waveguide, the device having a monolayer of a transition metal dichalcogenide disposed between the test cavity and the test microring resonator;
obtaining a sample of nasopharyngeal fluid from a subject being tested for COVID-19 infection on a swab;
suspending the swab in the test cavity formed in the surface of the silicon photonic biosensor device;
detecting the reference optical signal in the reference waveguide downstream from the reference cavity;
measuring the wavelength of the detected reference optical signal;
detecting the test optical signal in the test waveguide downstream from the test cavity;
measuring the wavelength of the detected test optical signal;
comparing the measured wavelength of the reference optical signal with the measured wavelength of the test optical signal to define any difference between the wavelengths;
diagnosing the subject as being infected with the COVID-19 infection when the difference between the wavelengths is greater than 0.01 nm; and
diagnosing the subject as being free of the COVID-19 infection when the difference between the wavelengths is less than 0.01 nm.

### Claim 14 (depends on 13)

The method for detecting COVID-19 infection according to claim 13, wherein the at least one antibody selectively binding to SARS-CoV-2 virus comprises at least one antibody selectively binding to the SARS-CoV-2 spike protein.

### Claim 15 (depends on 13)

The method for detecting COVID-19 infection according to claim 13, further comprising the steps of:
comparing the greater than 0.01 nm difference between the wavelengths to a reference chart plotting the difference between the wavelengths against number of days since exposure to the SARS-CoV-2 virus as determined by polymerase chain reaction (PCR); and
diagnosing the subject as being exposed to SARS-CoV-2 virus by the corresponding number of days shown on the reference chart.

### Claim 16 (depends on 13)

The method for detecting COVID-19 infection according to claim 13, further comprising the steps of:
comparing the greater than 0.01 nm difference between the wavelengths to a reference chart plotting the difference between the wavelengths against concentration of the SARS-CoV-2 virus as determined by polymerase chain reaction (PCR); and
diagnosing the subject as having a concentration of the SARS-CoV-2 virus by the corresponding concentration shown on the reference chart.