# US 11,079,360 B2 · Systems and methods for chromatographic analysis > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,079,360 B2 - Document ID: US11079360B2 - Status: Granted - Kind: B2 - Application number: 16/139,356 - Filed: 2018-09-24 - Published: 2019-04-04 - Granted: 2021-08-03 - Earliest claimed: 2017-10-04 - Assignees: Waters Technologies Corporation - Applicants: Waters Technologies Corporation - Inventors: Aaron D. Phoebe; Sylvain G. Cormier; Charles H. Phoebe, Jr. - Web page: https://patentgrep.com/patent/US11079360B2 ## CPC - G01N 30/20: using a sampling valve - G01N 30/32: of pressure or speed (G01N30/36 takes precedence) - G01N 30/64: Electrical detectors - G01N 30/8696: {Details of Software} - G01N 30/88: Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 - G01N 2030/027: {Liquid chromatography} - G01N 2030/201: {multiport valves, i.e. having more than two ports} - G01N 2030/202: {rotary valves} - G01N 2030/326: {pumps} - G01N 2030/328: {valves, e.g. check valves of pumps} - G01N 2030/8804: {automated systems} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract Methods of chromatographic detection are provided that include the step of providing a chromatography instrument without an autosampler, an external device, a pressurized sample injection device and a chromatographic data system. The chromatographic instrument has a sample valve, a column manager and a solvent manager. The present methods further include the steps: injecting sample into the sample valve with a pressurized sample injection device; transmitting a signal from the chromatographic data system to the external device, receiving the signal by the external device, producing an event output with the external device, discharging solvent from the solvent manager into the sample valve, and discharging sample into the column for chromatographic detection. ## Claims ### Claim 1 A method of chromatographic detection comprising the steps of: providing a liquid chromatography system comprising a chromatographic instrument having a sample valve, a column manager and a solvent manager; a chromatographic data system; an external device; and a pressurized sample injection device, wherein the sample valve is connected to the column manager, the solvent manager is connected to the column manager, and each of the column manager, the solvent manager, the external device and the chromatographic data system are in communication with the other; injecting sample into the sample valve with the pressurized sample injection device; transmitting a signal from the chromatographic data system to the external device; receiving the signal by the external device; producing an event output with the external device, wherein a fluidic pathway between the solvent manager and the column manager is established, and wherein the event output generates a contact closure that activates the sample valve; discharging solvent from the solvent manager into the sample valve; and discharging sample into the column for chromatographic detection. ### Claim 2 (depends on 1) The method of claim 1, further comprising the step of: transmitting an internal signal to the external device to trigger an Inject Start signal to the chromatographic instrument and chromatographic data system. ### Claim 3 (depends on 2) The method of claim 2, wherein the chromatographic instrument is started after an Inject Start signal is received by the solvent manager and the solvent manager signals the column manager to start. ### Claim 4 (depends on 1) The method of claim 1 wherein the sample valve moves from a first position to a second position establishing a fluidic pathway from the solvent manager to the column and sample is discharged from the sample valve to the column. ### Claim 5 (depends on 4) The method of claim 4, further comprising the step of resetting the sample valve to a first position wherein the sample valve is ready to be injected with another sample. ### Claim 6 (depends on 1) The method of claim 1, wherein the chromatographic instrument employs an liquid chromatography process, a high performance liquid chromatography process, an ultra performance convergence chromatography process, or an ultra high performance liquid chromatography process. ### Claim 7 (depends on 1) The method of claim 1, wherein sampling rates, BCD preferences, units and/or scale rates are associated with the external device. ### Claim 8 (depends on 1) The method of claim 1, wherein the chromatographic data system comprises a plurality of sample set methods for acquiring data. ### Claim 9 (depends on 1) The method of claim 1, wherein the chromatographic instrument further comprises a selector valve having a first position and a second position, wherein in the first position, the selector valve establishes a fluidic pathway between the sample valve and the column, and in the second position, the selector valve establishes a fluidic pathway between the column and a detector or a waste reservoir. ### Claim 10 (depends on 1) The method of claim 1, further comprising the step of discharging the sample from the column into a detector. ### Claim 11 (depends on 10) The method of claim 10, wherein the detector identifies and/or quantitates the concentration of sample constituents. ### Claim 12 A liquid chromatography system comprising: a chromatographic instrument having a sample valve, a column manager and a solvent manager; a chromatographic data system; an external device, wherein the external device further comprising a contact wherein the contact can activate and/or regulate the solvent manager and the column manager; and a pressurized sample injection device, wherein the sample valve is connected to the column manager, the solvent manager is connected to the column manager, and each of the column manager, the solvent manager, the external device and the chromatographic data system are in communication with the other, the external device comprising a single time base, dual-channel analog to digital converter configured to trigger an internal signal to start the chromatographic instrument and chromatographic data system. ### Claim 13 (depends on 12) The system of claim 12, wherein the chromatographic instrument employs an ultra-performance liquid chromatography process, a high performance liquid chromatography process, an ultra performance convergence chromatography process, or an ultra high performance liquid chromatography process. ### Claim 14 (depends on 12) The system of claim 12, wherein the sample valve is configured to move between a first position and a second position to establish a fluidic pathway from the solvent manager to the column. ### Claim 15 (depends on 12) The system of claim 12, wherein the sample valve is configured to move between the second position and the first position to reset the sample valve for a next injection of sample. ### Claim 16 (depends on 12) The system of claim 12, further comprising a detector wherein the detector identifies and/or quantitates the concentration of sample constituents. ### Claim 17 (depends on 16) The system of claim 16, further comprising a selector valve having a first position and a second position, wherein in the first position, the selector valve establishes a fluidic pathway between the sample valve and the column, and in the second position, the selector valve establishes a fluidic pathway between the column and a detector. ### Claim 18 (depends on 12) The system of claim 12, wherein the external device is configured to generate an event output to establish a fluidic pathway between the solvent manager and the column manager. ### Claim 19 A method of chromatographic detection comprising the steps of: providing a liquid chromatography system comprising a chromatographic instrument having a sample valve, a column manager and a solvent manager; a chromatographic data system; an external device, wherein the chromatographic data system comprises a plurality of sample set methods for acquiring data; and a pressurized sample injection device, wherein the sample valve is connected to the column manager, the solvent manager is connected to the column manager, and each of the column manager, the solvent manager, the external device and the chromatographic data system are in communication with the other; injecting sample into the sample valve with the pressurized sample injection device; transmitting a signal from the chromatographic data system to the external device; receiving the signal by the external device; producing an event output with the external device, wherein a fluidic pathway between the solvent manager and the column manager is established; discharging solvent from the solvent manager into the sample valve; and discharging sample into the column for chromatographic detection. ### Claim 20 (depends on 19) The method of claim 19, wherein the chromatographic instrument employs a liquid chromatography process, a high performance liquid chromatography process, an ultra performance convergence chromatography process, or an ultra high performance liquid chromatography process.