# US 11,078,546 B2 · Brassica GAT event and compositions and methods for the identification and/or detection thereof

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- Number: US 11,078,546 B2
- Document ID: US11078546B2
- Status: Granted
- Kind: B2
- Application number: 15/623,425
- Filed: 2017-06-15
- Published: 2017-12-21
- Granted: 2021-08-03
- Earliest claimed: 2010-11-24
- Assignees: E. I. Du Pont de Nemours and Company; Pioneer Hi-Bred International, Inc.
- Applicants: E I Du Pont De Nemours and Company; Pioneer Hi-Bred International, Inc.
- Inventors: David George Charne; Wenpin Chen; Chadwick Bruce Koscielny; Zhongsen Li; Jayantilal Devabhai Patel; Ferdinand Thoonen; Lomas Tulsieram; Yongping Zhang
- Web page: https://patentgrep.com/patent/US11078546B2

## CPC

- C12Q 1/6895: for plants, fungi or algae
- A01H 5/10: Seeds
- A01N 57/20: containing acyclic or cycloaliphatic radicals
- A23L 19/00: Products from fruits or vegetables; Preparation or treatment thereof (marmalades, jams, jellies or the like A23L21/10; treating harvested fruit or vegetables in bulk A23N)
- C07K 16/16: against material from plants
- C11B 1/00: Production of fats or fatty oils from raw materials
- C12N 15/8275: {Glyphosate}
- C12Q 2600/13: Plant traits
- G01N 33/0098: {Plants or trees (wood G01N33/46)}
- G01N 2021/8466: {Investigation of vegetal material, e.g. leaves, plants, fruits}
- G01N 2333/415: from plants
- G01N 2400/02: involving antibodies to sugar part of glycoproteins
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C11B: PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES (drying-oils C09F)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Compositions and methods related to transgenic glyphosate tolerant Brassica plants are provided. Specifically, the present invention provides Brassica plants having a DP-073496-4 event which imparts tolerance to glyphosate. The Brassica plant harboring the DP-073496-4 event at the recited chromosomal location comprises genomic/transgene junctions within SEQ ID NO: 2 or with genomic/transgene junctions as set forth in SEQ ID NO: 12 and/or 13. The characterization of the genomic insertion site of the event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event are provided.

## Claims

### Claim 1

A method of detecting the presence of DNA corresponding to a DP-073496-4 event in a sample, the method comprising:
(a) contacting the sample with a polynucleotide probe that hybridizes under stringent hybridization conditions with DNA from brassica event DP-073496-4, wherein the probe binds to a junction sequence of the event DP-073496-4, the junction sequence selected from the group consisting of SEQ ID NOS: 12 and 13;
(b) subjecting the sample and probe to stringent hybridization conditions; and
(c) detecting hybridization of the probe to the DNA, wherein detection of hybridization indicates the presence of the DP-073496-4 event.

### Claim 2 (depends on 1)

The method of claim 1, wherein said sample comprises brassica tissue.

### Claim 3 (depends on 1)

The method of claim 1, wherein the detection of hybridization indicates seed purity or seed lot purity.