Bioluminescent assays utilising secreted luciferases
AssigneeGene Stream Pty Ltd.
InventorsMarco Peter Leu, John Michael Daly
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Abstract
Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.
Claims12
A method for determining the amount or activity of at least one luciferase in a sample that comprises a recombinant luciferase that, in its wild type form, is a secreted luciferase, the method comprising:
- (i) contacting the sample with a composition comprising a reducing agent,
- (ii) contacting the sample with a composition comprising a luciferase substrate, and
- (iii) detecting bioluminescence in the sample.
Dependencies are inferred from the claim text.