Method for evaluating blood coagulation reaction
AssigneesChugai Seiyaku Kabushiki Kaisha, Public University Corporation Nara Medical University
InventorsTetsuhiro Soeda, Takehisa Kitazawa, Midori Shima
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Abstract
Various methods for evaluating hemostatic effect and various blood coagulation initiation reagents were studied to construct a method for evaluating blood coagulation reaction mediated by a substance having an activity of substituting for coagulation factor VIII (FVIII). As a result, it was discovered that by using a coagulation initiation reagent containing activated coagulation factor XI (FXIa) and phospholipids, the effect of a substance having an activity of substituting for coagulation factor VIII (FVIII) on blood coagulation reaction can be evaluated using the amount of thrombin generated in the blood sample as an indicator.
Claims8
A method for evaluating blood coagulation reaction mediated by a substance having an activity of substituting for coagulation factor VIII, wherein the method comprises:
- (i) adding a blood coagulation initiation reagent comprising activated coagulation factor XI and a phospholipid to a blood-derived sample isolated from a subject, wherein the coagulation initiation reagent does not comprise tissue factor, and wherein the sample comprises the substance having an activity of substituting for coagulation factor VIII;
- and
- (ii) measuring the amount of thrombin generated in the blood-derived sample obtained in (i),
- wherein the subject is a hemorrhagic disease patient,
- wherein the substance having the activity of substituting for coagulation factor VIII has been administered to the subject, and
- wherein the substance having the activity of substituting for coagulation factor VIII is a bispecific antibody that binds to coagulation factor IX or activated coagulation factor IX, and to coagulation factor X.
Dependencies are inferred from the claim text.