Methods for producing polypeptides in protease-deficient mutants of trichoderma

An isolated mutant of a parent Trichoderma strain, said parent strain comprising a first subtilisin-like serine protease gene encoding a first subtilisin-like serine protease, wherein the mutant strain is at least 95% deficient in the production of the first subtilisin-like serine protease compared to the parent Trichoderma strain when cultivated under identical conditions;

• wherein the first subtilisin-like serine protease is selected from the group consisting of:
• (a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
• 2 or at least 95% sequence identity to amino acids 20 to 882 of SEQ ID NO:
• 2, wherein said amino acid sequence has subtilisin-like serine protease activity;
• and
• (b) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to SEQ ID NO:
• 1 or at least 95% sequence identity to nucleotides 58 to 2774 of SEQ ID NO:
• 1, or the cDNA thereof, wherein the nucleotide sequence encodes an amino acid sequence having subtilisin-like serine protease activity;
• and
• wherein the mutant further comprises a polynucleotide encoding a polypeptide that is heterologous to the parent Trichoderma strain.