# US 11,078,505 B2 · Transaminase biocatalysts

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 11,078,505 B2
- Document ID: US11078505B2
- Status: Granted
- Kind: B2
- Application number: 16/807,977
- Filed: 2020-03-03
- Published: 2020-06-25
- Granted: 2021-08-03
- Earliest claimed: 2009-02-26
- Assignees: Codexis, Inc.
- Applicants: Codexis, Inc.
- Inventors: Christopher K. Savile; Emily Mundorff; Jeffrey C. Moore; Paul N. Devine; Jacob M. Janey
- Web page: https://patentgrep.com/patent/US11078505B2

## CPC

- C12P 17/182: {Heterocyclic compounds containing nitrogen atoms as the only ring heteroatoms in the condensed system (alloxazine or isoalloxazine, e.g. riboflavine C12P25/00)}
- C12N 9/1096: {transferring nitrogenous groups (2.6)}
- C12N 15/52: Genes encoding for enzymes or proenzymes
- C12P 13/001: {Amines; Imines}
- C12Y 206/01018: Beta-alanine-pyruvate transaminase (2.6.1.18)
- C12N 15/62: DNA sequences coding for fusion proteins
- Y02P 20/52: using catalysts, e.g. selective catalysts
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Y: ENZYMES
- Y02P: CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS

## Abstract

The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.

## Claims

### Claim 1

A recombinant transaminase polypeptide comprising an amino acid at least 95% sequence identical to SEQ ID NO: 2 having transaminase activity, wherein the amino acid sequence of the recombinant transaminase polypeptide comprises residue difference as compared to SEQ ID NO: 2 at residue positions X62, X69 and X169.

### Claim 2 (depends on 1)

The recombinant transaminase polypeptide of claim 1, wherein said recombinant transaminase polypeptide is capable of converting 4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro[1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl]-1-(2,4,5-trifluorophenyl)butan-2-one (“ketoamide substrate”) to (2R)-4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro[1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl]-1-(2,4,5-trifluorophenyl)butan-2-amine (“product”) under a defined reaction condition in the presence of an amino group donor isopropylamine to levels of the product detectable by HPLC-UV at 210 nm, where the reaction condition comprises about 2 g/L ketoamide substrate, about 0.5 M isopropylamine, about 22° C., about pH 7.5, about 5% DMSO, about 100 μM pyridoxal phosphate, and about 20 mg/mL of transaminase polypeptide.

### Claim 3 (depends on 1)

The recombinant transaminase polypeptide of claim 1, wherein said recombinant transaminase polypeptide is capable of converting the ketoamide substrate to the product with an activity that is equal to or greater than the activity of the polypeptide of SEQ ID NO: 4 under the defined reaction condition.

### Claim 4 (depends on 1)

The recombinant transaminase polypeptide of claim 1, wherein said recombinant transaminase polypeptide is capable of converting the ketoamide substrate to product in at least 90% enantiomeric excess.

### Claim 5 (depends on 1)

The recombinant transaminase polypeptide of claim 1, wherein said recombinant transaminase polypeptide is capable of converting the ketoamide substrate to product in at least 99% enantiomeric excess.