Yeast host cells for dicarboxylic acid production
AssigneeDsm IP Assets B.V.
InventorsAlrik Pieter Los, Zheng Zhao, René Verwaal, Jacobus Thomas Pronk, Rolf Poldermans
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Abstract
The present invention relates to a host cell which is capable of producing a dicarboxylic acid and which comprises at least one genetic modification in its genome resulting in the deficiency of at least one enzymatic step catalysing the oxidation of a cofactor. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting such a host cell in a suitable fermentation medium and producing the dicarboxylic acid.
Claims15
A yeast host cell which is capable of producing a dicarboxylic acid and which comprises a genetic modification in its genome resulting in the deficiency of
- mitochondrial external NADH dehydrogenase;
- mitochondrial glycerol-3-phosphate dehydrogenase;
- and
- cytosolic glycerol-3-phosphate dehydrogenase,
- wherein the genetic modification results in reduced oxidation of a reduced form of NADH, NADPH or FADH2 as compared to the oxidation of NADH, NADPH or FADH2 in a parent host cell that has not been modified in its genome in the same way,
- and wherein the host cell overexpresses a fumarase in E.C. 4.2.1.2 or a fumarate reductase in E.C. 1.3.1.6.
Dependencies are inferred from the claim text.