# US 11,078,503 B2 · Method for producing 3-hydroxyadipic acid

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- Number: US 11,078,503 B2
- Document ID: US11078503B2
- Status: Granted
- Kind: B2
- Application number: 16/304,916
- Filed: 2017-05-30
- Published: 2019-09-12
- Granted: 2021-08-03
- Earliest claimed: 2016-05-31
- Assignees: Toray Industries, Inc.
- Applicants: Toray Industries, Inc.
- Inventors: Kyohei Isobe; Kenji Kawamura; Masateru Ito; Katsushige Yamada
- Web page: https://patentgrep.com/patent/US11078503B2

## CPC

- C12P 7/44: Polycarboxylic acids
- C12N 1/20: Bacteria; Culture media therefor
- C12N 1/38: Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound (C12N1/34 takes precedence)
- C12N 15/52: Genes encoding for enzymes or proenzymes
- C12R 2001/425: Serratia
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

Provided is a method of producing 3-hydroxyadipic acid, the method including the step of culturing a microorganism belonging to the genus Serratia capable of producing 3-hydroxyadipic acid.

## Claims

### Claim 1

A microorganism belonging to the genus Serratia producing 3-hydroxyadipic acid, wherein an enzymatic activity that catalyzes a reaction to produce 3-oxoadipyl-CoA and CoA from succinyl-CoA and acetyl-CoA is enhanced by introducing a gene encoding a protein having said enzymatic activity to said microorganism belonging to the genus Serratia, or by modifying a functional region(s) in a vicinity of a coding region in a gene encoding a protein having said enzymatic activity in said microorganism belonging to the genus Serratia.

### Claim 2 (depends on 1)

The microorganism belonging to the genus Serratia according to claim 1, wherein said microorganism belonging to the genus Serratia is Serratia grimesii, Serratia ficaria, Serratia fonticola, Serratia odorifera, Serratia plymuthica, Serratia entomophila, or Serratia nematodiphila.

### Claim 3 (depends on 1)

A method of producing 3-hydroxyadipic acid, said method comprising the step of culturing a microorganism claimed in claim 1 belonging to the genus Serratia capable of producing 3-hydroxyadipic acid.

### Claim 4 (depends on 3)

The method of producing 3-hydroxyadipic acid according to claim 3, wherein said microorganism belonging to the genus Serratia has an enhanced enzymatic activity that catalyzes a reaction to produce 3-oxoadipyl-CoA and CoA from succinyl-CoA and acetyl-CoA.

### Claim 5 (depends on 3)

The method of producing 3-hydroxyadipic acid according to claim 3, wherein said microorganism belonging to the genus Serratia is Serratia grimesii, Serratia ficaria, Serratia fonticola, Serratia odorifera, Serratia plymuthica, Serratia entomophila, or Serratia nematodiphila.

### Claim 6 (depends on 3)

The method of producing 3-hydroxyadipic acid according to claim 3, wherein a medium for culturing said microorganism belonging to the genus Serratia contains at least one or more carbon sources selected from the group consisting of saccharides, succinic acid, 2-oxoglutaric acid, and glycerol.

### Claim 7 (depends on 3)

The method of producing 3-hydroxyadipic acid according to claim 3, wherein said microorganism belonging to the genus Serratia is cultured in a medium that contains at least one or more inducers selected from the group consisting of ferulic acid, p-coumaric acid, benzoic acid, cis,cis-muconic acid, protocatechuic acid, and catechol.