US 11,078,502 B2 · Method and cell line for production of polyketides in yeast

Abstract

A method and cell line for producing polyketides in yeast. The method applies, and the cell line includes, a yeast cell transformed with a polyketide synthase coding sequence. The polyketide synthase enzyme catalyzes synthesis of olivetol or methyl-olivetol, and may include Dictyostelium discoideum polyketide synthase (“DiPKS”). Wild type DiPKS produces methyl-olivetol only. DiPKS may be modified to produce olivetol only or a mixture of both olivetol and methyl-olivetol. The yeast cell may be modified to include a phosphopantethienyl transferase for increased activity of DiPKS. The yeast cell may be modified to mitigate mitochondrial acetaldehyde catabolism for increasing malonyl-CoA available for synthesizing olivetol or methyl-olivetol.

Claims40

Dependent claims

A yeast cell for producing at least one species of polyketide, the yeast cell comprising a first polynucleotide coding for a D. discoideum polyketide synthase (DiPKS) enzyme, which produces at least one species of polyketide from malonyl-CoA, the polyketide having structure I:

wherein, on structure I, R1 is an alkyl group having 5 carbons, and R2 and R3 are both H, or are methyl and H;
and
wherein the DiPKS polyketide synthase enzyme has a primary structure with between 99% and 100% amino acid residue sequence identity with a protein coded for by a reading frame defined by bases 523 to 9966 of SEQ ID NO:
13, with a charged amino acid residue at position 1516 in place of a glycine residue at position 1516;
and
the at least one species of polyketide comprises a first polyketide wherein R2 is H and R3 is H.

3 more elements

Dependencies are inferred from the claim text.

Method and cell line for production of polyketides in yeast

Life of this patent

Method and cell line for production of polyketides in yeast

Life of this patent

Abstract

Claims40