Nucleosidase
AssigneesAmano Enzyme Inc., Suntory Holdings Limited
InventorsKeita Okuda, Toru Katase, Yuko Kuritani, Naoki Matsumoto, Tomoko Fujimura, Ikuma Mizuguchi, Takako Inui
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Abstract
It is an object of the present invention to provide a novel enzyme useful for producing low-purine foods or beverages. There is disclosed a nucleosidase comprising an amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having 85% or more identity with the amino acid sequence, or an amino acid sequence of SEQ ID NO: 2 or an amino acid sequence having 88% or more identity with the amino acid sequence.
Claims8
A recombinant DNA comprising any DNA selected from the group consisting of the following (a) to (d):
- (a) a DNA encoding the amino acid sequence of SEQ ID No:
- 1 or SEQ ID No:
- 2;
- (b) a DNA encoding an amino acid sequence having 85% or more identity with the amino acid sequence of SEQ ID No:
- 1 or an amino acid sequence having 88% or more identity with the amino acid sequence of SEQ ID No:
- 2 and encoding a protein having nucleosidase activity;
- (c) a DNA consisting of the base sequence of SEQ ID No:
- 3, or SEQ No:
- 5;
- and
- (d) a DNA having a base sequence equivalent to the base sequence of SEQ ID No:
- 3, equivalent to the base sequence of SEQ ID No:
- 4, equivalent to the base sequence of SEQ No:
- 5, or equivalent to the base sequence of SEQ ID No:
- 6, and wherein the DNA encodes a protein having nucleosidase activity.
A method for producing a nucleosidase, comprising the following steps (1) and (2):
- (1) culturing a microorganism comprising a nucleosidase gene;
- wherein the encoded nucleosidase comprises the amino acid sequence of SEQ ID No:
- 1 or an amino acid sequence having 85% or more identity with the amino acid sequence of SEQ ID No:
- 1, or the amino acid sequence of SEQ ID No:
- 2 or an amino acid sequence having 88% or more identity with the amino acid sequence of SEQ ID No:
- 2 and
- (2) collecting the nucleosidase from the culture solution and/or the cell bodies after culture.
A method for producing a nucleosidase preparation, comprising the following steps (I) and (II):
- (I) culturing a microorganism comprising a nucleosidase gene, wherein the encoded nucleosidase comprises the amino acid sequence of SEQ ID No:
- 1 or an amino acid sequence having 85% or more identity with the amino acid sequence of SEQ ID No:
- 1, or the amino acid sequence of SEQ ID No:
- 2 or an amino acid sequence having 88% or more identity with the amino acid sequence of SEQ ID No:
- 2;
- and
- (II) removing the cell bodies after culture.
Dependencies are inferred from the claim text.