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US 11,078,458 B2Granted
Genome-wide rationally-designed mutations leading to enhanced lysine production in E. coli
AssigneeInscripta, Inc.
InventorsRichard Fox, Daniel Held, Eric Abbate, Michael Clay, Katherine Krouse, Nandini Krishnamurthy, Krishna Yerramsetty
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Abstract
The present disclosure relates to various different types of variants in E. coli coding and noncoding regions leading to enhanced lysine production for, e.g., supplements and nutraceuticals.
Claims24
An engineered E. coli cell comprising the following variant sequences:
- a promoter sequence having the nucleic acid SEQ ID NO:
- 2 driving transcription of a dapA gene, and further comprising one of the following proteins expression of which is not driven by a promoter sequence having a nucleic acid of SEQ ID NO:
- 2:
- a mfdY5 protein having the amino acid sequence of SEQ ID NO:
- 21, a nupXR5 protein having the amino acid sequence of SEQ ID NO:
- 22, a pck protein having the amino acid sequence of SEQ ID NO:
- 23, a rlmL protein having the amino acid sequence of SEQ ID NO:
- 26, a wzxB protein having the amino acid sequence of SEQ ID NO:
- 27, a ytfP protein having the amino acid sequence of SEQ ID NO:
- 33, a marA protein having the amino acid sequence of SEQ ID NO:
- 34, a marA protein having the amino acid sequence of SEQ ID NO:
- 35, a metL protein having the amino acid sequence of SEQ ID NO:
- 36, a metL protein having the amino acid sequence of SEQ ID NO:
- 37, a pck protein having the amino acid sequence of SEQ ID NO:
- 38.
12 more elements
An engineered E. coli cell comprising the following variant sequences:
- a promoter sequence having the nucleic acid SEQ ID NO:
- 2 driving transcription of a dapA gene, and further comprising one of the following:
- a promoter sequence having the nucleic acid sequence of SEQ ID NO:
- 24 driving expression of a phoB protein;
- a promoter sequence having the nucleic acid sequence of SEQ ID NO:
- 25 driving expression of a purM protein;
- a promoter sequence having the nucleic acid sequence of SEQ ID NO:
- 28 driving expression of a ydgl protein;
- a promoter sequence having the nucleic acid sequence of SEQ ID NO:
- 29 driving expression of a ydgE protein;
- a promoter sequence having the nucleic acid sequence of SEQ ID NO:
- 30 driving expression of a yicL protein;
- a promoter sequence having the nucleic acid sequence of SEQ ID NO:
- 31 driving of a yliE protein;
- a promoter sequence having the nucleic acid sequence of SEQ ID NO:
- 32 driving expression of a yohF protein;
- a promoter sequence having the nucleic acid sequence of SEQ ID NO:
- 39 driving expression of a setB protein;
- a promoter sequence having the nucleic acid sequence of SEQ ID NO:
- 40 driving expression of a ydfO protein;
- a promoter sequence having the nucleic acid sequence of SEQ ID NO:
- 41 driving expression of a ydgD protein;
- or a promoter sequence having the nucleic acid sequence of SEQ ID NO:
- 42 driving expression of a yejD protein.
21 more elements
Dependencies are inferred from the claim text.