# US 11,078,457 B2 · Lysinibacillus fusiformis with methylamine degradability and application thereof

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- Number: US 11,078,457 B2
- Document ID: US11078457B2
- Status: Granted
- Kind: B2
- Application number: 16/491,919
- Filed: 2018-03-20
- Published: 2020-12-03
- Granted: 2021-08-03
- Earliest claimed: 2017-11-01
- Assignees: Guangdong University of Technology
- Applicants: Guangdong University of Technology
- Inventors: Guiying Li; Shiai Li; Taicheng An; Yuetan Su; Xun'an Ning
- Web page: https://patentgrep.com/patent/US11078457B2

## CPC

- B09C 1/00: Reclamation of contaminated soil
- C12N 1/20: Bacteria; Culture media therefor
- B01D 53/84: Biological processes
- B09C 1/002: {involving in-situ ground water treatment}
- B09C 1/10: microbiologically, {biologically} or by using enzymes
- B09C 1/105: {using fungi or plants}
- C02F 3/32: characterised by the animals or plants used, e.g. algae
- C02F 3/341: {Consortia of bacteria}
- B01D 2251/95: Specific microorganisms
- B01D 2257/40: Nitrogen compounds
- B01D 2257/70: Organic compounds not provided for in groups B01D2257/00 - B01D2257/602
- B01D 2258/06: Polluted air
- B09C 2101/00: In situ
- C12N 1/205: Bacterial isolates
- C12R 2001/01: Bacteria or Actinomycetales {; using bacteria or Actinomycetales}
- Y02A 50/20: Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
- B09C: RECLAMATION OF CONTAMINATED SOIL (gatherers for removing stone or the like from the soil A01B43/00; sterilising soil by steam A01G11/00; removing undesirable matter, e.g. rubbish, from the land E01H15/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28)
- C02F: TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE (separation in general B01D; special arrangements on waterborne vessels of installations for treating water, waste water or sewage, e.g. for producing fresh water, B63J; adding materials to water to prevent corrosion C23F; treating radioactively-contaminated liquids G21F9/04; regeneration of reactants for recirculation into processes, see the relevant places for the processes)
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

The present invention discloses a strain of Lysinibacillus fusiformis with methylamine degradability and the application thereof. This strain, named Lysinibacillus fusiformis GDUTAN2, was deposited on May 24, 2017 in the China Center for Type Culture Collection in Wuhan University, Wuhan City, Hubei Province with a deposit number of CCTCC NO. M 2017284. This Lysinibacillus fusiformis GDUTAN2 was Grain-positive and rod-shaped, and the colony appeared to be round, white and transparent, having a diameter of 1-2 mm. The Lysinibacillus fusiformis GDUTAN2 of the present invention can be applied to environmental restoration, degrading methylamine in the environment at a high degradation efficiency. When it degraded methylamine for 96 h at a substrate concentration of 130 mg/L, the degradation efficiency could reach 32.8%.

## Claims

### Claim 1

A method for degrading methylamine, comprising:
a) applying an effective amount of composition comprising Lysinibacillus fusiformis GDUTAN2 to a substrate containing methylamine, wherein the Lysinihaciiius fusiformis GDUTAN2 was deposited on May 24, 2017 in the China Center for Type Culture Collection in Wuhan University Wuhan City, Hubei Province with a deposit number of CCTCC NO. M 2017284; and
b) contacting the substrate with the composition comprising L. fusiformis GDUTAN2 for a sufficient length of time to degrade the methylamine.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the 16S rDNA sequence of the Lysinibacillus fusiformis GDUTAN2 is set forth in SEQ ID NO:1.

### Claim 3 (depends on 1)

The method according to claim 1, wherein the substrate includes atmosphere, water or soil.

### Claim 4 (depends on 2)

The method according to claim 2, wherein the substrate includes atmosphere, water or soil.