# US 11,078,456 B2 · Fusarium solani and use of same in prevention and treatment of southern blight of Dendrobium officinale Kimura et Migo

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- Number: US 11,078,456 B2
- Document ID: US11078456B2
- Status: Granted
- Kind: B2
- Application number: 16/650,975
- Filed: 2018-08-13
- Published: 2020-09-10
- Granted: 2021-08-03
- Earliest claimed: 2017-09-30
- Assignees: Jiangsu Vocational College of Agriculture and Forestry
- Applicants: Jiangsu Vocational College of Agriculture and Forestry
- Inventors: Chao Xu; Hongyan Zhang; Hetong Yang; Gangjun Xi; Kai Zheng; Jun Shi
- Web page: https://patentgrep.com/patent/US11078456B2

## CPC

- C12N 1/14: Fungi (culture of mushrooms A01G18/00; as new plants A01H15/00); Culture media therefor
- A01N 63/30: Microbial fungi; Substances produced thereby or obtained therefrom
- C12N 1/145: Fungi isolates
- C12R 2001/77: Fusarium
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

A Fusarium solani strain and a use thereof in the control of southern blight disease of Dendrobium officinale Kimura et Migo, and the strain JSNL007-2 has been identified as Fusarium solani and has been deposited in China Center for Type Culture Collection on Mar. 10, 2017 with a deposit number of CGMCC NO. 13687. The Fusarium solani JSNL007-2 of the present invention has a significant effect on the disease resistance of Dendrobium officinale Kimura et Migo, especially for improving the ability of Dendrobium officinale Kimura et Migo to resist southern blight disease, and has a certain effect on reducing the use of chemical pesticides, which is an important means to ensure food safety.

## Claims

### Claim 1

A method for improving the resistance of Dendrobium officinale Kimura et Migo plants to southern blight disease comprising a step of administrating an effective amount of a microbial inoculant comprising Fusarium solani to a Dendrobium officinale Kimura et Migo plant subject in need of improving resistance to southern blight disease.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the plant subject is a surface of a Dendrobium officinale Kimura et Migo seedling, and the effective amount of the microbial inoculant is 5-8 grams per the Dendrobium officinale Kimura et Migo seedling.

### Claim 3 (depends on 2)

The method according to claim 2, wherein relative water content of the plant is maintained above 60% for 6-10 days after the microbial inoculant is administrated on the surface of the Dendrobium officinale Kimura et Migo seedling.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the Fusarium solani is Fusarium solani strain JSNL007-2, which has been deposited in China Center for Type Culture Collection on Mar. 10, 2017 with a deposit number of CGMCC NO. 13687.