# US 11,078,248 B2 · BMP peptides and methods of use

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- Number: US 11,078,248 B2
- Document ID: US11078248B2
- Status: Granted
- Kind: B2
- Application number: 16/218,979
- Filed: 2018-12-13
- Published: 2019-05-23
- Granted: 2021-08-03
- Earliest claimed: 2010-03-19
- Assignees: LifeNet Health
- Applicants: LifeNet Health
- Inventors: Xiaofei Qin; Silvia Chen; Jingsong Chen; James Clagett
- Web page: https://patentgrep.com/patent/US11078248B2

## CPC

- C07K 14/51: Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
- A61K 38/1875: {Bone morphogenic factor; Osteogenins; Osteogenic factor; Bone-inducing factor}
- A61K 38/48: acting on peptide bonds (3.4)
- C12N 5/0602: {Vertebrate cells}
- C12N 5/0654: {Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth}
- C12N 5/0655: {Chondrocytes; Cartilage}
- C12N 5/0658: {Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts}
- C12N 9/48: acting on peptide bonds (3.4)
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- C07K 2319/50: containing protease site
- C12N 2501/155: Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
- C12N 2506/1323: from skeletal muscle cells
- C12N 2510/00: Genetically modified cells
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The invention relates to truncated growth factors and variants thereof. The invention also relates to methods of making and using the truncated growth factors. The invention further relates to compositions including a protease and a growth factor comprising a bone morphogenic protein (BMP) or a variant thereof. The invention also relates to methods of using the composition.

## Claims

### Claim 1

A method of promoting differentiation of cells into osteoblasts or chondrocytes, the method comprising treating the cells with a peptide consisting of the amino acid sequence of SEQ ID NO: 19.

### Claim 2 (depends on 1)

The method of claim 1, wherein the cells are progenitor cells or adult stem cells.

### Claim 3 (depends on 2)

The method of claim 2, wherein the progenitor cells or the adult stem cells are derived from placenta, bone marrow, adipose tissue, blood vessel, amniotic fluid, synovial fluid, synovial membrane, pericardium, periosteum, dura, peripheral blood, umbilical blood, menstrual blood, baby teeth, nucleus pulposus, brain, skin, hair follicle, intestinal crypt, neural tissue, or muscle.

### Claim 4 (depends on 1)

The method of claim 1, wherein the cells are induced pluripotent stem cells.

### Claim 5

A method of promoting osteogenesis of cells in a tissue, the method comprising treating the tissue with
a peptide consisting of the amino acid sequence of SEQ ID NO: 19.

### Claim 6 (depends on 5)

The method of claim 5, wherein the osteogenic activity of cells in the treated tissue is greater than the osteogenic activity of cells in untreated tissue.

### Claim 7 (depends on 5)

The method of claim 5, wherein the tissue is bone tissue or connective tissue.