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US 11,078,237 B2Granted
Antigen delivery platforms
AssigneeGlaxosmithkline Biologicals SA
InventorsMichael Franti, Anders Lilja, Rebecca Loomis, Peter W. Mason
Life of this patent
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Abstract
This disclosure provides platforms for delivery of herpes virus proteins to cells, particularly proteins that form complexes in vivo. In some embodiments these proteins and the complexes they form elicit potent neutralizing antibodies. Thus, presentation of herpes virus proteins using the disclosed platforms permits the generation of broad and potent immune responses useful for vaccine development.
Claims19
A self-replicating RNA molecule comprising a polynucleotide which comprises:
- a) a first nucleotide sequence encoding a first protein or fragment thereof from cytomegalovirus (CMV), wherein the first nucleotide sequence is operably linked to a subgenomic promoter and followed by b);
- b) a second nucleotide sequence encoding a second protein or fragment thereof from said CMV, wherein the second nucleotide sequence is operably linked to a subgenomic promoter and followed by c);
- c) a third nucleotide sequence encoding a third protein or fragment thereof from said CMV, wherein the third nucleotide sequence is operably linked to a subgenomic promoter and followed by d);
- d) a fourth nucleotide sequence encoding a fourth protein or fragment thereof from said CMV, wherein the fourth nucleotide sequence is operably linked to an IRES or a viral 2A site and followed by e);
- and
- e) a fifth nucleotide sequence encoding a fifth protein or fragment thereof from said CMV, wherein the fifth nucleotide sequence is operably linked to an IRES or a viral 2A site,
- wherein the first protein is gH, the second protein is gL, the third protein is UL128, the fourth protein is UL130, and the fifth protein is UL131;
- and wherein introducing the self-replicating RNA molecule into a suitable cell results in the expression of the first, second, third, fourth and fifth CMV proteins or fragments thereof in an amount sufficient for the formation of a gH/gL/UL128/UL130/UL131 pentameric complex.
5 more elements
Dependencies are inferred from the claim text.