# US 11,077,476 B2 · Methods and compositions for In Situ groundwater remediation

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- Number: US 11,077,476 B2
- Document ID: US11077476B2
- Status: Granted
- Kind: B2
- Application number: 15/988,284
- Filed: 2018-05-24
- Published: 2018-12-20
- Granted: 2021-08-03
- Earliest claimed: 2017-05-24
- Assignees: Mork Technologies, LLC
- Applicants: Benjamin V. Mork
- Inventors: Benjamin V. Mork
- Web page: https://patentgrep.com/patent/US11077476B2

## CPC

- B09C 1/002: {involving in-situ ground water treatment}
- B09C 1/08: chemically
- B09C 1/10: microbiologically, {biologically} or by using enzymes
- C02F 1/68: by addition of specified substances, e.g. trace elements, for ameliorating potable water (medicinal water A61K)
- C02F 3/342: {characterised by the enzymes used}
- B09C 2101/00: In situ
- C02F 1/283: {using coal, charred products, or inorganic mixtures containing them}
- C02F 2103/06: Contaminated groundwater or leachate
- B09C: RECLAMATION OF CONTAMINATED SOIL (gatherers for removing stone or the like from the soil A01B43/00; sterilising soil by steam A01G11/00; removing undesirable matter, e.g. rubbish, from the land E01H15/00)
- C02F: TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE (separation in general B01D; special arrangements on waterborne vessels of installations for treating water, waste water or sewage, e.g. for producing fresh water, B63J; adding materials to water to prevent corrosion C23F; treating radioactively-contaminated liquids G21F9/04; regeneration of reactants for recirculation into processes, see the relevant places for the processes)

## Abstract

Groundwater, slurry, or soil treatment or remediation compositions and methods of both production and use are described that include: at least one colloidal remediation chemical, at least one polysaccharide stabilizer, and at least one enzyme.

## Claims

### Claim 1

A method of treating or remediating groundwater or soil, comprising:
providing at least one colloidal remediation chemical,
providing at least one polysaccharide stabilizer,
providing at least one zone of untreated groundwater or untreated soil, at least one zone of contaminated groundwater or contaminated soil, or a combination thereof, wherein the at least one zone has a water source;
depositing the at least one colloidal remediation chemical and the at least one polysaccharide stabilizer in at least part of the at least one zone of untreated groundwater or untreated soil, at least one zone of contaminated groundwater or contaminated soil, or a combination thereof;
allowing the deposited composition to interact with the at least one zone of untreated groundwater or untreated soil, at least one zone of contaminated groundwater or contaminated soil, or a combination thereof; and
adding at least one enzyme to the deposited composition, wherein the enzyme embeds and immobilizes at least part of the deposited composition in the at least one zone of untreated groundwater or untreated soil, at least one zone of contaminated groundwater or contaminated soil, or a combination thereof.

### Claim 2 (depends on 1)

The method of claim 1, wherein the enzyme embeds and immobilizes at least part of the at least one colloidal remediation chemical in the at least one zone of untreated groundwater or untreated soil, at least one zone of contaminated groundwater or contaminated soil, or a combination thereof.

### Claim 3 (depends on 1)

The method of claim 1, wherein the groundwater treatment or remediation composition further comprises water.

### Claim 4 (depends on 1)

The method of claim 1, wherein the at least one colloidal remediation chemical comprises about 0.01 to about 50 weight percent of the composition.

### Claim 5 (depends on 4)

The method of claim 4, wherein the at least one colloidal remediation chemical comprises about 0.01 to about 5 weight percent of the composition.

### Claim 6 (depends on 1)

The method of claim 1, wherein the at least one polysaccharide stabilizer comprises about 0.01 to about 20 weight percent of the composition.

### Claim 7 (depends on 6)

The method of claim 6, wherein the at least one polysaccharide stabilizer comprises about 0.01 to about 5 weight percent of the composition.

### Claim 8 (depends on 1)

The method of claim 1, wherein the at least one enzyme comprises about 0.001 to about 5 weight percent of the composition.

### Claim 9 (depends on 8)

The method of claim 8, wherein the at least one enzyme comprises about 0.001 to about 2 weight percent of the composition.

### Claim 10 (depends on 1)

The method of claim 1, wherein the at least one colloidal remediation chemical comprises at least one reducing agent, at least one electron acceptor, at least one catalyst, at least one sorbent material, at least one metal-binding agent, at least one microbial culture, or a combination thereof.

### Claim 11 (depends on 1)

The method of claim 1, wherein the at least one enzyme comprises a cellulase enzyme.

### Claim 12 (depends on 10)

The method of claim 10, wherein the at least one reducing agent comprises at least one electron donor.

### Claim 13 (depends on 1)

The method of claim 1, wherein the at least one colloidal remediation chemical comprises at least one oxidant.
