# US 11,077,235 B2 · Fat-depleted adipose tissue and a device and method for preparing the same > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,077,235 B2 - Document ID: US11077235B2 - Status: Granted - Kind: B2 - Application number: 15/571,432 - Filed: 2016-05-05 - Published: 2019-01-10 - Granted: 2021-08-03 - Earliest claimed: 2015-05-05 - Assignees: Samer Srouji; Zohar Gendler - Applicants: Samer Srouji; Zohar Gendler - Inventors: Samer Srouji - Web page: https://patentgrep.com/patent/US11077235B2 ## CPC - A61K 35/28: Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells - A61K 35/32: Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane - A61K 35/35: Fat tissue; Adipocytes; Stromal cells; Connective tissues (adipose-derived stem cells A61K35/28; collagen A61K38/39) - A61M 1/604: {Bag or liner in a rigid container, with suction applied to both} - A61M 1/80: {Suction pumps (A61M1/64, A61M1/71, A61M60/00 take precedence)} - B30B 9/04: using press rams - B30B 9/06: co-operating with permeable casings or strainers - B30B 15/08: Accessory tools, e.g. knives; Mountings therefor - B30B 15/22: controlling the degree of pressure applied by the ram during the pressing stroke - A61M 1/892: {with treatment of the collected fat} - A61M 2202/08: Lipoids - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61M: DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY (introducing media into or onto the bodies of animals A61D7/00; means for inserting tampons A61F13/26; devices for administering food or medicines orally A61J; containers for collecting, storing or administering blood or medical fluids A61J1/05); DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY (surgery A61B; chemical aspects of surgical articles A61L); DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR {(Electrotherapy, e.g. producing anaesthesia by the use of alternating or intermittent currents A61N1/36021)} - B30B: PRESSES IN GENERAL (producing ultra-high pressure or ultra-high pressure and high temperature to effect modifications of a substance, e.g. for making artificial diamonds B01J3/00 ){(compressing means for refuse receptacles B65F1/1405)} ## Abstract A device configured to mechanically remove a fat phase from at least one adipose tissue includes a first pressing element and a second pressing element. Furthermore, there is provided a method for preparing a preparation including a fat-depleted adipose tissue, the method including mechanically removing a fat phase from at least one adipose tissue. In addition, there is provided a preparation including a fat-depleted adipose tissue, wherein the fat-depleted adipose tissue is prepared by mechanically removing a fat phase from at least one adipose tissue. ## Claims ### Claim 1 A device configured to mechanically remove a fat phase from at least one adipose tissue and prepare adipose derived mesenchymal stem cells, the device comprising: a first pressing element and a second pressing element, for pressing the at least one adipose tissue therebetween and forcing out at least part of the fat phase from the at least one adipose tissue when the pressing elements are moved toward one another; and a distance controlling element configured to maintain a gap between the first pressing element and the second pressing element during pressing of the at least one adipose tissue to avoid over-pressing or crushing of the at least one adipose tissue to prevent damage to the adipose derived mesenchymal stem cells, wherein the size of the gap is controlled to be correlated to the size of the at least one adipose tissue pressed therebetween, and wherein the gap has a size of less than 500 μm. ### Claim 2 (depends on 1) The device according to claim 1, further comprising a fat phase separating element. ### Claim 3 (depends on 2) The device according to claim 2, wherein the fat phase separating element is a sucking element. ### Claim 4 (depends on 2) The device according to claim 2, wherein the fat phase separating element is a drainage element. ### Claim 5 (depends on 1) The device according to claim 1, further comprising at least one perforating element attached to one of the first pressing element, the second pressing element, or to both pressing elements, wherein the at least one perforating element is configured to perforate the at least one adipose tissue placed in the gap and facilitate exit of the fat phase from the at least one adipose tissue. ### Claim 6 (depends on 1) The device according to claim 1, wherein at least one of the pressing elements is substantially flat. ### Claim 7 (depends on 1) The device according to claim 1, further comprising a temperature control element configured to control a temperature under which the at least one adipose tissue is pressed. ### Claim 8 (depends on 7) The device according to claim 7, wherein the temperature is in the range of substantially 37-42° C. ### Claim 9 (depends on 1) The device according to claim 1, wherein the first pressing element is stationary. ### Claim 10 (depends on 5) The device according to claim 5, wherein the at least one perforating element comprises at least one tooth-like structure that is attached to a surface of the first pressing element, or the second pressing element, or both the first pressing element and the second pressing element. ### Claim 11 (depends on 1) The device according to claim 1, wherein the first pressing element has a bowl-like structure having a space, and the second pressing element has a bowl-like structure that is configured to be accommodated in the space of the first bowl-like pressing element. ### Claim 12 (depends on 1) The device according to claim 1, further comprising a pressing and perforating unit configured to be provided between the first pressing element and the second pressing element, wherein the pressing and perforating unit comprises: a frame having a first surface facing the gap and configured to be in contact with the at least one adipose tissue, wherein the frame encloses a lattice of a plurality of crossed strips defining a plurality of lattice spaces; and at least one perforating member attached to the cross strips and configured to extend from the lattice spaces to beyond the first surface so as to perforate the at least one adipose tissue that is placed on top of the first surface. ### Claim 13 (depends on 12) The device according to claim 12, wherein the perforating member comprising: a tip pointing toward the gap; a leg attached to the tip, and configured to connect to one of the pressing elements; and a resilient connector connecting the tip to the strip so as to allow movements of the tip from the lattice spaced to beyond the first surface and vice versa.