# US 11,077,226 B2 · Scalable three-dimensional elastic construct manufacturing > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,077,226 B2 - Document ID: US11077226B2 - Status: Granted - Kind: B2 - Application number: 16/937,442 - Filed: 2020-07-23 - Published: 2020-11-05 - Granted: 2021-08-03 - Earliest claimed: 2012-12-10 - Assignees: Allergan Pharmaceuticals International Limited - Applicants: Allergan Pharmaceuticals International Limited - Inventors: Anthony Steven Weiss; Suzanne Marie Mithieux - Web page: https://patentgrep.com/patent/US11077226B2 ## CPC - A61L 27/22: Polypeptides or derivatives thereof {, e.g. degradation products} - A61L 27/227: {Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24} - A61L 27/52: Hydrogels or hydrocolloids - B05D 3/007: {After-treatment} - C07K 14/78: Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG] - C09D 189/00: Coating compositions based on proteins; Coating compositions based on derivatives thereof - A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) - A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K) - B05D: PROCESSES FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL (conveying articles or workpieces through baths of liquid B65G, e.g. B65G49/02) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - C09D: COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR (cosmetics A61K; processes for applying liquids or other fluent materials to surfaces, in general, B05D; staining wood B27K5/02; glazes or vitreous enamels C03C; natural resins, French polish, drying-oils, driers, turpentine, per se, C09F; polishing compositions other than French polish, ski waxes C09G; adhesives or use of materials as adhesives C09J; materials for sealing or packing joints or covers C09K3/10; materials for stopping leaks C09K3/12; processes for the electrolytic or electrophoretic production of coatings C25D) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) ## Abstract Tissue repair and restoration can be performed using an elastic material formed from tropoelastin. The elastic material can be formed by providing a solution of tropoelastin monomers, applying the solution to a surface, and heating the solution on the surface in absence of a cross-linking agent to enable the tropoelastin monomers to bind to each other to form an elastic material that does not dissociate into tropoelastin monomers when the elastic material is contacted with an aqueous solution. ## Claims ### Claim 1 A method for forming an elastic material, including: applying tropoelastin monomers to a surface; and heating the tropoelastin monomers on the surface to a temperature of from about 60° C. to about 200° C. at a pH of less than 8.5 and in the absence of a cross-linking agent to enable the tropoelastin monomers to bind to each other to form an elastic material that does not dissociate into tropoelastin monomers when the elastic material is contacted with an aqueous solution, wherein the elastic material is hard and glassy. ### Claim 2 (depends on 1) The method of claim 1, wherein the tropoelastin monomers are heated to a temperature that is sufficient to enable the tropoelastin monomers to bind to each other to form an elastic material that does not dissociate into tropoelastin monomers when the elastic material is exposed to physiological conditions. ### Claim 3 (depends on 1) The method of claim 1, where in the tropoelastin monomers are heated to a temperature that is sufficient to enable the tropoelastin monomers to bind to each other to form an elastic material that does not dissociate into tropoelastin monomers when the elastic material is contacted with an aqueous solution having a pH of from about 6.5 to 8.0. ### Claim 4 (depends on 1) The method of claim 1, where in the tropoelastin monomers are heated to a temperature that is sufficient to enable the tropoelastin monomers to bind to each other to form an elastic material that does not dissociate into tropoelastin monomers when the elastic material is contacted with an aqueous solution having a temperature of from about 30 to about 45° C. ### Claim 5 (depends on 1) The method of claim 1, wherein the tropoelastin monomers are heated to a temperature that is sufficient to enable the tropoelastin monomers to bind to each other to form an elastic material that does not dissociate into tropoelastin monomers when the elastic material is contacted with an aqueous solution having a salt concentration of about 75 mM to about 300 mM. ### Claim 6 (depends on 1) The method of claim 1, where in the surface is heated for heating of the tropoelastin monomers. ### Claim 7 (depends on 1) The method of claim 1, wherein the tropoelastin monomers contain hydrophilic and hydrophobic domains of tropoelastin. ### Claim 8 (depends on 1) The method of claim 1, where in the tropoelastin monomers have a sequence that has at least 90% sequence identity with an amino acid sequence of human tropoelastin across at least 50 consecutive amino acids. ### Claim 9 (depends on 1) The method of claim 1, where in the tropoelastin monomers are recombinant tropoelastin monomers having an amino acid sequence of a human tropoelastin isoform. ### Claim 10 (depends on 1) The method of claim 1, wherein the tropoelastin monomers are applied to the surface by spraying the tropoelastin monomers onto the surface. ### Claim 11 (depends on 1) The method of claim 1, wherein the surface is provided in the form of a die, mold or cast enabling the elastic material formed by the method to be shaped into a predefined shape. ### Claim 12 A method for forming an elastic material, the method comprising: applying tropoelastin monomers to a surface; and heating the tropoelastin monomers on the surface to a temperature of from about 60° C. to about 200° C. in the absence of a cross-linking agent to enable the tropoelastin monomers to bind to each other to form a hard, elastic material that does not dissociate into tropoelastin monomers when the elastic material is contacted with an aqueous solution. ### Claim 13 A method for forming an elastic material, the method comprising: applying tropoelastin monomers to a surface; and heating the tropoelastin monomers on the surface to a temperature of from about 60° C. to about 200° C. in the absence of a cross-linking agent to enable the tropoelastin monomers to bind to each other to form a glassy, elastic material that does not dissociate into tropoelastin monomers when the elastic material is contacted with an aqueous solution. ### Claim 14 (depends on 13) The method of claim 13, wherein the elastic material has an increased crystallinity after heating. ### Claim 15 A method for forming an elastic material, the method comprising: applying tropoelastin monomers to a surface; and heating the tropoelastin monomers on the surface to a temperature of from about 60° C. to about 200° C. in the absence of a cross-linking agent to enable the tropoelastin monomers to bind to each other to form a glassy, elastic material that does not substantially dissociate into tropoelastin monomers when the elastic material is contacted with an aqueous solution. ### Claim 16 (depends on 15) The method of claim 15, wherein the tropoelastin monomers on the surface are heated to a temperature of from about 130° C. to about 170° C. ### Claim 17 (depends on 15) The method of claim 15, wherein the tropoelastin monomers on the surface are heated to a temperature of from about 140° C. to about 160° C. ### Claim 18 (depends on 15) The method of claim 15, wherein the tropoelastin monomers on the surface are heated to a temperature of about 160° C.