# US 11,077,154 B2 · Probiotic composition and uses thereof

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- Number: US 11,077,154 B2
- Document ID: US11077154B2
- Status: Granted
- Kind: B2
- Application number: 16/099,987
- Filed: 2017-05-09
- Published: 2019-05-09
- Granted: 2021-08-03
- Earliest claimed: 2016-05-09
- Assignees: Deerland Probiotics & Enzymes A/S
- Applicants: Deerland Probiotics & Enzymes A/S
- Inventors: Erik Brandsborg
- Web page: https://patentgrep.com/patent/US11077154B2

## CPC

- A61K 35/747: Lactobacilli, e.g. L. acidophilus or L. brevis
- A61P 1/02: Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
- C12N 1/20: Bacteria; Culture media therefor
- C12N 1/205: Bacterial isolates
- C12R 2001/225: Lactobacillus
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

The present invention relates to a Lactobacillus curvatus strain. In particular, the present invention relates to a composition comprising a Lactobacillus curvatus strain in combination with a further Lactobacillus and the use of the same in the treatment of inflammatory conditions in the oral cavity.

## Claims

### Claim 1

A Lactobacillus curvatus strain EB10 (DSM 32307), wherein said strain is in a lyophilized or spray dried form.

### Claim 2 (depends on 1)

A culture comprising the Lactobacillus curvatus strain EB10 (DSM 32307) of claim 1, wherein said culture is in a lyophilized or spray dried form.

### Claim 3 (depends on 2)

The culture according to claim 2, further comprising at least one additional Lactobacillus sp.

### Claim 4 (depends on 1)

A composition comprising the Lactobacillus curvatus strain EB10 (DSM 32307) of claim 1.

### Claim 5 (depends on 4)

The composition according to claim 4, further comprising at least one additional Lactobacillus sp.

### Claim 6 (depends on 4)

The composition according to claim 4, wherein said composition comprises from 105 to 1013 CFU of the Lactobacillus curvatus strain EB10 (DSM 32307) of said composition.

### Claim 7 (depends on 5)

The composition according to claim 5, wherein said composition comprises at least 107 CFU Lactobacillus curvatus strain EB10 (DSM 32307) per gram of said composition and at least 107 CFU Lactobacillus rhamnosus strain PB01 (DSM 14870) per gram of said composition.

### Claim 8 (depends on 4)

The composition according to claim 4, wherein said composition is a tablet, capsule, powder, effervescent tablet, effervescent powder, granulate, microencapsulated product, suspension, spray, gel or cream.

### Claim 9 (depends on 4)

A method of inhibiting inflammation in the oral cavity of a subject comprising administering the composition of claim 4 to said subject.

### Claim 10 (depends on 9)

The method according to claim 9, wherein said inflammation is presented in said subject as gingivitis or periodontitis.

### Claim 11 (depends on 9)

The method according to claim 9, wherein said composition comprises from 107 to 1010 CFU Lactobacillus curvatus strain EB10 (DSM 32307) per daily dose.

### Claim 12 (depends on 9)

The method according to claim 9, wherein said composition comprises at least 5×107 CFU Lactobacillus curvatus strain EB10 (DSM 32307) per daily dose and at least 5×107 CFU Lactobacillus rhamnosus strain PB01 (DSM 14870) per daily dose.

### Claim 13 (depends on 4)

The composition according to claim 4, wherein said composition is a nutraceutical or nutritional supplement.

### Claim 14 (depends on 4)

A method for maintaining the oral health in a subject, said method comprising providing the composition according to claim 4 to said subject.

### Claim 15 (depends on 3)

The culture of claim 3, wherein the at least one additional Lactobacillus sp. is Lactobacillus rhamnosus strain PB01 (DSM 14870).

### Claim 16 (depends on 5)

The composition of claim 5, wherein the at least one additional Lactobacillus sp. is Lactobacillus rhamnosus strain PB01 (DSM 14870).

### Claim 17 (depends on 4)

The composition according to claim 4, wherein said composition comprises at least one excipient.

### Claim 18 (depends on 17)

The composition according to claim 17, wherein said at least one excipient is selected from the group consisting of a bulking agent, a binder, a glazing agent, a sweetener and a flavour.

### Claim 19 (depends on 18)

The composition according to claim 18, wherein said bulking agent is at least one bulking agent selected from the group consisting of xylitol, sorbitol, erythritol, maltitol, lactitol, inositol and mannitol microcrystalline cellulose and isomalt.

### Claim 20 (depends on 18)

The composition according to claim 18, wherein said binder is at least one binder selected from the group consisting of maltodextrin and sodium carboxymethylcellulose.

### Claim 21 (depends on 18)

The composition according to claim 18, wherein said glazing agent is at least one glazing agent selected from the group consisting of mono- and diglyceride of fatty acids, silicon dioxide, stearic acid, beeswax, candelilla wax, carnauba wax, shellac, microcrystalline wax, crystalline wax, lanolin, oxidized polyethylene wax, esters of colophonium, and paraffin.

### Claim 22 (depends on 18)

The composition according to claim 18, wherein said sweetener is at least one sweetener selected from the group consisting of stevia, a steviol glycoside such as stevioside and rebaudioside, aspartame, sucralose, neotame, acesulfame potassium (Ace-K), saccharin, and advantame.

### Claim 23 (depends on 18)

The composition according to claim 18, wherein said flavour is at least one flavour selected from the group consisting of citric acid, lemon flavour, honeydew melon flavour, blueberry flavour, peach flavour, strawberry flavour, raspberry flavour, cola flavour, chocolate flavour, peppermint flavour, cherry flavour, lime flavour, orange flavour, vanilla flavour, tangerine flavour, liquorice flavour, apricot flavour, eucalyptus flavour, green tea flavour, ginger flavour and bilberry flavour.