# US 11,076,786 B2 · Wound monitoring sensors and use thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,076,786 B2 - Document ID: US11076786B2 - Status: Granted - Kind: B2 - Application number: 16/589,437 - Filed: 2019-10-01 - Published: 2020-04-02 - Granted: 2021-08-03 - Earliest claimed: 2018-10-01 - Assignees: The Florida International University Board of Trustees; University of Miami - Applicants: Sohini Roy Choudhury; Yogeswaran Umasankar; Shekhar Bhansali; Robert S. Kirsner; Hadar A. Lev-Tov - Inventors: Sohini Roy Choudhury; Yogeswaran Umasankar; Shekhar Bhansali; Robert S. Kirsner; Hadar A. Lev-Tov - Web page: https://patentgrep.com/patent/US11076786B2 ## CPC - A61B 5/1486: using enzyme electrodes, e.g. with immobilised oxidase - A61B 5/445: {Evaluating skin irritation or skin trauma, e.g. rash, eczema, wound, bed sore} - A61B 5/14507: {specially adapted for measuring characteristics of body fluids other than blood (devices for taking samples of body liquids A61B10/0045)} - A61B 5/14517: {for sweat} - A61B 5/14539: {for measuring pH} - A61B 5/14546: {for measuring analytes not otherwise provided for, e.g. ions, cytochromes} - A61B 5/20: for measuring urological functions {restricted to the evaluation of the urinary system (A61B5/4375 takes precedence)} - A61B 5/4266: {sweat secretion} - A61B 5/4277: {saliva secretion} - A61B 5/6833: {Adhesive patches} - A61B 2560/0412: Low-profile patch shaped housings - A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D) ## Abstract The subject invention provides sensor systems that can detect biomarkers related to wound healing (e.g., uric acid, adenosine, arginine and/or xanthine). In one embodiment, the subject invention pertains to materials and methods for monitoring biomarkers non-invasively in a wound and a biofluid (e.g., sweat) in the proximity of the wound, optionally, including other physiological fluids. Skin based, non-invasive enzymatic electrochemical biosensor on a wearable platform (e.g., sweat patch) that can evaluate the healing of wounds through assessment of its biomarker levels are provided. This non-invasive detection from physiologically biofluids can reduce or eliminate occlusion effects. ## Claims ### Claim 1 A flexible enzymatic electrochemical sensor comprising a working electrode, a reference electrode and a counter electrode, the working electrode being functionalized with an enzyme, the enzyme being selected from uricase (UOx), adenosine deaminase, arginase and xanthine oxidase (XO), the working electrode being further functionalized with a polymer and catalytic metal nano-structures selected from nano-flakes, nano-rods, and nano-wires. ### Claim 2 (depends on 1) The flexible enzymatic electrochemical sensor of claim 1, further comprising an electrode transfer mediator. ### Claim 3 (depends on 2) The flexible enzymatic electrochemical sensor of claim 2, the electrode transfer mediator being ferrocene carboxylic acid (FCA). ### Claim 4 (depends on 1) The flexible enzymatic electrochemical sensor of claim 1, the electrode being further deposited with a nano-structure. ### Claim 5 (depends on 4) The flexible enzymatic electrochemical sensor of claim 4, the nano-structure comprising multi-walled carbon nanotubes (MWCNTs). ### Claim 6 (depends on 1) The flexible enzymatic electrochemical sensor of claim 1, the polymer being Poly (vinyl alcohol) N-methyl-4(4′-formylstyryl)-pyridinium-metho-sulfate-acetal (PVA-SbQ). ### Claim 7 (depends on 1) The flexible enzymatic electrochemical sensor of claim 1, the enzyme being entrapped in the polymer with a polymer to enzyme ratio (v/v) of from 1:1 to 1:6. ### Claim 8 (depends on 1) The flexible enzymatic electrochemical sensor of claim 1, the working electrode being made of gold, silver, platinum or carbon. ### Claim 9 (depends on 1) The flexible enzymatic electrochemical sensor of claim 1, the working electrode being a carbon electrode functionalized with uricase (UOx), an ionic polymer and catalytic Ag nano-wires, UOx being entrapped in the ionic polymer, and the flexible enzymatic electrochemical sensor further comprising an electrode transfer mediator comprising FCA. ### Claim 10 (depends on 1) The flexible enzymatic electrochemical sensor of claim 1, the enzyme being adenosine deaminase. ### Claim 11 (depends on 1) The flexible enzymatic electrochemical sensor of claim 1, the enzyme being arginase. ### Claim 12 (depends on 1) The flexible enzymatic electrochemical sensor of claim 1, the working electrode being a carbon electrode functionalized with xanthine oxidase (XO), and MWCNTs on the surface. ### Claim 13 (depends on 1) A method for monitoring the healing of a wound comprising contacting a wound care substrate with a wound of a subject, the wound care substrate comprising a flexible enzymatic electrochemical sensor of claim 1; and measuring an electrical signal generated from a reaction between the flexible enzymatic electrochemical sensor and a biomarker in the wound, the biomarker being uric acid, adenosine, arginine and/or xanthine. ### Claim 14 (depends on 13) The method of claim 13, comprising applying the wound care substrate onto the wound, applying the wound care substrate in the vicinity of the wound, embedding the wound care substrate in the wound or a wound extract, contacting the wound care substrate with a biofluid of the wound, and/or embedding the wound care substrate in the biofluid of the wound. ### Claim 15 (depends on 14) The method of claim 14, the biofluid being sweat, plasma, blood, urine, tear, saliva, or serum. ### Claim 16 (depends on 1) A method for assessing wound severity comprising contacting a wound care substrate with a wound of a subject, the wound care substrate comprising a flexible enzymatic electrochemical sensor of claim 1; and measuring an electrical signal generated from a reaction between the flexible enzymatic electrochemical sensor and a biomarker in the wound, the biomarker being uric acid, adenosine, arginine and/or xanthine. ### Claim 17 A flexible enzymatic electrochemical sensor, comprising a working electrode, a reference electrode and a counter electrode, the working electrode being a carbon electrode functionalized with uricase (UOx), PVA-SbQ, and catalytic nano-wires on the surface, UOx being entrapped in the matrix of PVA-SbQ, and the flexible enzymatic electrochemical sensor further comprising FCA.