# US 11,076,602 B2 · Mummified mealy bug product and method > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,076,602 B2 - Document ID: US11076602B2 - Status: Granted - Kind: B2 - Application number: 16/710,960 - Filed: 2019-12-11 - Published: 2020-04-16 - Granted: 2021-08-03 - Earliest claimed: 2011-05-11 - Assignees: Bio-Bee Sde Eliyahu Ltd. - Applicants: Bio-Bee Sde Eliyahu Ltd. - Inventors: Shimon Steinberg; Eyal Erel - Web page: https://patentgrep.com/patent/US11076602B2 ## CPC - A01N 63/14: Insects - A01N 63/00: Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates (containing compounds of determined constitution A01N27/00 - A01N59/00; unicellular algae A01N65/03) - A01N 63/10: Animals; Substances produced thereby or obtained therefrom - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS ## Abstract A Biological Control Agent (BCA) system comprising processed parasitized mealybug mummies is disclosed. The processed and modified parasitized mealybug mummies are essentially devoid of their limbs and waxy covering. In the novel BCA system the modified parasitized mealybug mummies are processed in a manner that strips off their waxy covering and detaches their limbs without impacting on their viability as a host for parasitoid pupae. The modified parasitized mealybug mummies of the BCA system are encased in specially adapted packaging for delivering and distributing the modified parasitized mealybug mummies to plants with mealybug infestations. A method of preparing and storing the modified parasitized mealybug mummies as a BCA is also presented. ## Claims ### Claim 1 A process of producing parasitized mealybug mummies as a mealybug Biological Control Agent (BCA), the process comprising the steps of: a) infesting a host plant with 1st instar stage mealybugs; b) detaching mealybug nymphs and young females from said host plant; c) parasitizing said detached mealybug nymphs and young females; d) incubating the parasitized mealybugs to produce a mummified parasitized mealybug mixture; e) rinsing the mummified parasitized mealybug mixture; f) separating out viable mummified parasitized mealybugs from said mixture; g) drying the viable mummified parasitized mealybugs; h) packaging said viable mummified parasitized mealybugs, wherein said packaged mummified parasitized mealybugs are devoid of limbs and waxy covering; and i) storing and/or distributing said packaged mummified parasitized mealybugs as a mealybug BCA. ### Claim 2 (depends on 1) The process according to claim 1, wherein the packaged mummified parasitized mealybugs are viable for at least 14 days. ### Claim 3 (depends on 1) The process according to claim 1, wherein said mummified parasitized mealybugs are derived from a mealybug species selected from the group consisting of Planococcus citri, Planococcus ficus, Planococcus vovae, Phenacoccus solani, Phenacoccus madeirensis Phenacoccus herreni, Pseudococcus comstocki, Pseudococcus cryptus, Pseudococcus viburni, Pseudococcus longispinus Dysmicoccus brevipes, Maconellicoccus hirsutus or a combination thereof. ### Claim 4 (depends on 1) The process according to claim 1, wherein said parasitoid is selected from the group of consisting of endo-parasitic wasps, Encyrtidae family, Leptomastix algirica, Anagyrus pseudococci or a combination thereof. ### Claim 5 (depends on 1) The process of claim 1, further comprising controlling mealybug infestations, by administering the mealybug BCA (a) to a crop at risk of infestation by mealybugs, or (b) to an infested crop. ### Claim 6 (depends on 5) The process of controlling mealybug infestations according to claim 5, wherein the mealybug BCA can be stored for up to 18 days before administering the mealybug BCA. ### Claim 7 (depends on 1) The process according to claim 1, wherein said step of infesting comprises an additional step of maintaining said 1st instar stage mealybugs for about 25 days at about 25° C., and at about 60% relative humidity, on a factitious plant host, until they reach the 3rd instar nymph or young female stage. ### Claim 8 (depends on 1) The process according to claim 1, wherein said step of infesting comprises an additional step of maintaining said 1st instar stage mealybugs for between about 35 to 42 days at about 27° C., and at about 60% relative humidity on a factitious plant host, until they reach the 3rd instar nymph or young female stage. ### Claim 9 (depends on 7) The process according to claim 7, wherein the factitious plant host is a potato sprout. ### Claim 10 (depends on 1) The process according to claim 1, wherein said step of detaching comprises an additional step of blowing mealybug nymphs or young females from their host plant using an air blower. ### Claim 11 (depends on 1) The process according to claim 1, wherein said step of detaching comprises an additional step of blowing 3rd instar mealybug nymphs or young females from their host plant using an air blower. ### Claim 12 (depends on 1) The process according to claim 1, wherein said step of parasitizing mealybug nymphs and young females comprises parasitizing mealybug 3rd instar nymphs and young females. ### Claim 13 (depends on 1) The process according to claim 1, wherein said step of parasitizing mealybug nymphs and young females comprises separating dirt and small mealybugs from larger mealybugs through a sieving tower. ### Claim 14 (depends on 1) The process according to claim 1, wherein said step of parasitizing mealybug nymphs and young females comprises exposing weighed and quantified mealybug nymphs and young females to a prospective parasitoid population in a parasitization cage. ### Claim 15 (depends on 1) The process according to claim 1, wherein the step of incubating the parasitized mealybugs comprises an additional step of incubating the parasitized mealybugs to produce the mummified parasitized mealybug mixture in a parasitization cage. ### Claim 16 (depends on 15) The process according to claim 15, wherein the step of incubating the parasitized mealybugs comprises an additional step of removing parasitized mealybugs from said parasitization cage to be incubated until the parasitoids reach the pupal or pre-pupal stage, while replenishing the parasitization cage with a fresh unparasitized mealybug population. ### Claim 17 (depends on 1) The process according to claim 1, wherein said step of rinsing parasitized mealybugs comprises the additional step of rinsing parasitized mealybugs that have reached the parasitoid's pupal or pre-pupal stage through a sieving tower with water spray nozzles, thereby separating out large viable mealybug mummies from dead or small mealybugs. ### Claim 18 (depends on 1) The process according to claim 1, wherein said step of separating out viable parasitized mealybugs mummies comprises the additional step of selecting floating mealybugs and mummies from mixtures that have been drained in a conical water bath. ### Claim 19 (depends on 1) The process according to claim 1, wherein said packaging step comprises at least one additional step selected from the group consisting of: a) weighing and quantifying said parasitized mealybug mummies by counting samples under a stereoscopic microscope; b) providing a quantified population of viable parasitized mealybug mummies for storage or distribution; and c) mixing parasitized mealybug mummies with fine sawdust in a 5:1 ratio, respectively, in a ventilated and labeled plastic container in preparation for storage at 14° C. ### Claim 20 (depends on 1) The process according to claim 1, wherein the mealybug BCA is packaged in a sealed ventilated container adapted to be attached to a plant or an object adjoining a crop and function as an effective distribution means of the mealybug BCA at a desired location.