# US 11,076,573 B2 · Endophytic microbial symbionts in plant prenatal care > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,076,573 B2 - Document ID: US11076573B2 - Status: Granted - Kind: B2 - Application number: 16/141,859 - Filed: 2018-09-25 - Published: 2019-03-21 - Granted: 2021-08-03 - Earliest claimed: 2013-02-05 - Assignees: University of Saskatchewan - Applicants: University Of Saskatchewan - Inventors: Vladimir Vujanovic; James J. Germida - Web page: https://patentgrep.com/patent/US11076573B2 ## CPC - A01H 17/00: Symbiotic or parasitic combinations including one or more new plants, e.g. mycorrhiza (lichens A01H15/00) - A01H 3/00: Processes for modifying phenotypes {, e.g. symbiosis with bacteria} (A01H4/00 takes precedence) - A01N 25/00: Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application {, e.g. seed treatment or sequential application}; Substances for reducing the noxious effect of the active ingredients to organisms other than pests - A01N 63/00: Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates (containing compounds of determined constitution A01N27/00 - A01N59/00; unicellular algae A01N65/03) - A01N 63/28: Streptomyces - A01N 63/30: Microbial fungi; Substances produced thereby or obtained therefrom - B09C 1/105: {using fungi or plants} - C12N 1/145: Fungi isolates - C12N 1/205: Bacterial isolates - B09C 2101/00: In situ - C12R 2001/465: Streptomyces - C12R 2001/645: Fungi {; Processes using fungi} - C12R 2001/80: Penicillium - A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - B09C: RECLAMATION OF CONTAMINATED SOIL (gatherers for removing stone or the like from the soil A01B43/00; sterilising soil by steam A01G11/00; removing undesirable matter, e.g. rubbish, from the land E01H15/00) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS ## Abstract The present disclosure provides compositions comprising novel endophytes capable of promoting germination endophytes that have a symbiotic relationship with plants. The present disclosure further provides methods of improving seed vitality, biotic and abiotic stress resistance, plant health and yield under both stressed and unstressed environmental conditions, comprising inoculating a seed with the novel endophyte strains and cultivating a plant therefrom. ## Claims ### Claim 1 A method of increasing resistance to nitrogen stress in at least one agricultural crop, said method comprising mechanically or manually inoculating a seed of the agricultural crop with a composition comprising at least one endophyte and a carrier, wherein the at least one endophyte is of genus Streptomyces and comprises a 16S rDNA sequence with 100% sequence identity to SEQ ID NO:6 or is deposited as IDAC 081111-06 and is capable of capable of causing populations of seeds to have increased yield under nitrogen stress conditions compared to control seeds. ### Claim 2 (depends on 1) The method of claim 1, wherein the endophyte is deposited as IDAC 081111-06. ### Claim 3 (depends on 1) The method of claim 1, wherein the endophyte comprises a 16S rDNA sequence with 100% sequence identity to SEQ ID NO:6. ### Claim 4 (depends on 1) The method of claim 1, wherein the agricultural crop is selected from the group consisting of cereal, pulse, and canola. ### Claim 5 (depends on 1) The method of claim 1, wherein the crop is a cereal. ### Claim 6 (depends on 1) The method of claim 1, wherein the crop is wheat. ### Claim 7 (depends on 1) The method of claim 1, wherein the seed is a durum wheat cultivar AC Avonlea seed. ### Claim 8 (depends on 1) The method of claim 1, wherein the nitrogen stress conditions result from lack of application of nitrogen to a field. ### Claim 9 (depends on 1) The method of claim 1, wherein the nitrogen stress conditions result from field nitrogen not present at agriculturally relevant rates. ### Claim 10 (depends on 1) The method of claim 1, wherein manually or mechanically inoculating the seed comprises coating the seed with the endophyte. ### Claim 11 (depends on 1) The method of claim 1, wherein the inoculated seed is coated with at least 100 CFU of the endophyte per seed. ### Claim 12 (depends on 1) The method of claim 1, wherein the carrier is a liquid carrier. ### Claim 13 (depends on 1) The method of claim 1, wherein the endophyte colonizes at least 1% of the cortical cells of an agricultural plant grown from the inoculated seed. ### Claim 14 (depends on 1) The method of claim 1, wherein the endophyte is a spore. ### Claim 15 (depends on 1) The method of claim 1, further comprising cultivating the inoculated seed into a first generation plant. ### Claim 16 (depends on 1) The method of claim 1, wherein the endophyte is deposited as IDAC 081111-06, the crop is wheat, and manually or mechanically inoculating the seed comprises coating the seed with the endophyte.