DNA methylation and genotype specific biomarker for predicting post-traumatic stress disorder
AssigneesThe Johns Hopkins University, UMC Utrecht Holding B.V.
InventorsZachary Kaminsky, Marco Paul Maria Boks
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Abstract
The present invention relates to the field of biomarkers. More specifically, the present invention relates to the use of biomarkers to predict post-traumatic stress disorder (PTSD). In one embodiment, a method for predicting PTSD in a subject comprises the steps of (a) measuring the DNA methylation level of a CpG dinucleotide in the 3′ untranslated region of SKA2; (b) identifying the genotype at a SNP within the 3′ UTR of SKA2, and (c) predicting PTSD in the subject using a prediction algorithm.
Claims14
A method for predicting post-traumatic stress disorder (PTSD) in a subject comprising the steps of:
- (a) measuring the DNA methylation level of a CpG located on the minus strand of chromosome 17, at position 57187729, from DNA isolated from a sample collected from the subject, wherein the DNA methylation level is measured using a primer comprising SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 or SEQ ID NO:5;
- (b) detecting the genotype at the single nucleotide polymorphism (SNP), rs7208505, from DNA isolated from a sample collected from the subject;
- and
- (c) using a linear model that utilizes the DNA methylation level, genotype at rs7208505, age and sex to predict PTSD in the subject.
A method for predicting PTSD comprising the steps of:
- (a) measuring DNA methylation level at a CpG dinucleotide located in the 3′ untranslated region (UTR) of SKA2 from DNA isolated from a sample collected from the subject, wherein the DNA methylation level is measured using a primer comprising SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 or SEQ ID NO:5;
- (b) detecting the genotype at the SNP rs7208505, from DNA isolated from a sample collected from the subject;
- and
- (c) using a linear model that incorporates the measured DNA methylation level and genotype to predict PTSD in the subject.
Dependencies are inferred from the claim text.