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US 11,072,649 B2Granted
Systems and methods for the production of human polyclonal antibodies
AssigneeSab, LLC
InventorsJay Hooper, Eddie Sullivan, Hua Wu
Life of this patent
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Abstract
Disclosed herein is a method for producing human antibodies against a pathogen comprising injecting a non-human animal with a pathogen-derived DNA vaccine in at least two locations of the animal; injecting the animal with an adjuvant in a location of the animal different from the location of the DNA vaccine location; collecting plasma from the animal after the injections; and purifying polyclonal antibody from the plasma.
Claims13
A method for producing human antibodies against a viral pathogen-derived DNA vaccine, comprising:
- a) injecting at least two DNA vaccines in at least two separate locations of a transchromosomal ungulate, the at least two DNA vaccines comprising:
- (i) a first DNA vaccine encoding a first antigen from a first strain of a viral pathogen, and
- (ii) a second DNA vaccine encoding a second antigen from a second strain of the same viral pathogen,
- wherein the first antigen is injected at a first location and the second antigen is injected at a second location, wherein each of the first and second locations is on a separate quarter of the transchromosomal ungulate;
- wherein the transchromosomal ungulate produces fully human IgG antibodies, wherein the transchromosomal ungulate is a knock-out for endogenous ungulate immunoglobulin genes, and comprises a human artificial chromosome (HAC) containing the full germ line sequence of human immunoglobulins;
- b) injecting an adjuvant to the transchromosomal ungulate in a location different from the first DNA vaccine location and the second DNA vaccine location;
- c) collecting plasma from the transchromosomal ungulate;
- and
- d) purifying polyclonal human IgG antibodies from the plasma.
6 more elements
Dependencies are inferred from the claim text.