Soybean ROD1 gene sequences and uses thereof

A method for determining the presence or absence of a knock-out allele of a ROD1 gene in a biological sample, comprising

• providing genomic DNA from said biological sample, and
• analyzing said genomic DNA for the presence of at least one molecular marker linked to said knock-out allele of the ROD1 gene by
• amplifying at least a portion of said genomic DNA in a polymerase chain reaction (PCR) assay, wherein the portion comprises the molecular marker, or
• contacting the genomic DNA with a nucleic acid probe, under high stringency conditions that allow hybridization of the probe with a portion of the genomic DNA encoding the molecular marker;
• wherein said knock-out allele of the ROD1 gene encodes a protein having at least 90% sequence identity to SEQ ID No. 3 or SEQ ID No. 6, and
• wherein the molecular marker is a stop codon or a splice site mutation in said knock-out allele of the ROD1 gene,
• wherein the at least one molecular marker comprises a stop codon mutation at position 2947-2949, 3049-3051, 3088-3090, 3495-3497, 3513-3515, 3519-3521, 3600-3602, 3615-3617, 4410-4412, 4416-4418, 4428-4430, and/or 4455-4457 of SEQ ID NO:
• 1, or at position 3013-3015, 3103-3105, 3142-3144, 3530-3532, 3548-3550, 3554-3556, 3635-3637, 4077-4079, 4191-4193, 4197-4199, 4209-4211, 4236-4238, and/or 4380-4382 of SEQ ID NO:
• 4, or
• wherein the at least one molecular marker comprises a splice site mutation at position 3264, 3265, 3479, 3480, 3617, 3618, 4298, and/or 4299 of SEQ ID NO:
• 1, or position 3318, 3319, 3514, 3515, 3652, 3653, 4079, and/or 4080 of SEQ ID NO:
• 4.