RNA polymerase variants
AssigneeModernaTX, Inc.
InventorsAmy E. Rabideau, Athanasios Dousis, Kanchana Ravichandran, Elissa Hobert
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Abstract
The present disclosure provides, in some aspects, variant RNA polymerases, the use of which increases transcription efficiency while reducing the number of double-stranded RNA contaminates and run-on transcripts produced during an in vitro transcription reaction.
Claims13
A method of performing an in vitro transcription (IVT) reaction, comprising combining a deoxyribonucleic acid (DNA) with a T7 ribonucleic acid (RNA) polymerase, nucleoside triphosphates and buffer, wherein the T7 RNA polymerase variant comprises an amino acid sequence having at least 90% identity to the amino acid sequence of SEQ ID NO:
- 1 modified to comprise an amino acid substitution at position 47, and the T7 RNA polymerase variant has RNA polymerase activity.
A method of performing an in vitro transcription (IVT) reaction, comprising combining a deoxyribonucleic acid (DNA) with a T7 ribonucleic acid (RNA) polymerase, nucleoside triphosphates and buffer, wherein the T7 RNA polymerase variant comprises an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO:
- 1 modified to comprise an alanine at position 47, and the T7 RNA polymerase variant has RNA polymerase activity.
Dependencies are inferred from the claim text.