Production of itaconic acid
AssigneeLesaffre Et Compagnie
InventorPeter Jan Punt
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Abstract
The invention relates to a method to increase production of itaconic acid in a micro-organism by inhibiting the expression or functioning of the enzyme itaconyl-CoA transferase, itaconyl-CoA hydratase (citramalyl-CoA hydrolyase; EC 4.2.1.56) and/or citramalyl-CoA lyase. Also embodied are micro-organisms, preferably Aspergillus terreus or Aspergillus niger, in which said enzyme is inhibited.
Claims15
A method to increase production of itaconic acid in a micro-organism, comprising:
- (i) culturing a micro-organism selected from the group of strains as deposited under no. CBS 141661 and CBS 141662 with the Westerdijk Fungal Biodiversity Institute in a suitable medium;
- (ii) inhibiting expression or function of itaconyl-CoA transferase (EC 2.8.3.-), itaconyl-CoA hydratase (citramalyl-CoA hydro-lyase;
- EC 4.2.1.56), citramalyl CoA lyase (EC 4.1.3.25), or a combination thereof in the micro-organism;
- and providing the micro-organism with a gene encoding for the enzyme 2-methylcitrate dehydratase, wherein the production of itaconic acid is increased at least 2 fold compared to a parental micro-organism.
A method for producing itaconic acid in a micro-organism, comprising culturing a micro-organism selected from the group of strains as deposited under no. CBS 141661 and CBS 141662 with the Westerdijk Fungal Biodiversity Institute in a suitable medium, wherein the production of itaconic acid is increased at least 2 fold compared to a parental micro-organism, thereby producing itaconic acid and optionally isolating said itaconic acid from the medium.
Dependencies are inferred from the claim text.