PH20 polypeptide variants, formulations and uses thereof

A modified PH20 polypeptide, comprising one or more amino acid replacements in an unmodified PH20 polypeptide, wherein:

• the unmodified PH20 polypeptide consists of the amino acid sequence selected from the group consisting of SEQ ID NOs:
• 3, 7 and 32-66;
• the modified PH20 polypeptide exhibits increased hyaluronidase activity compared to the unmodified PH20 polypeptide not containing the amino acid replacement(s);
• the amino acid replacement(s) confer(s) the increased hyaluronidase activity;
• an amino acid replacement that confers the increased hyaluronidase activity is at a position corresponding to residue 309, with reference to amino acid positions of SEQ ID NO:3;
• corresponding amino acid positions are identified by alignment of the PH20 polypeptide with the polypeptide having the sequence of amino acids of SEQ ID NO:3;
• and
• the modified PH20 polypeptide has at least 91% sequence identity to the amino acid sequence selected from the group consisting of SEQ ID NOs:
• 3, 7 and 32-66.