Immunogenic compositions containing bacterial outer membrane vesicles and therapeutic uses thereof
AssigneeBiomvis Srl
InventorsGuido Grandi, Valeria Cafardi, Laura Fantappie′, Renata Grifantini, Alberto Grandi
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Abstract
The present invention provides means and products for the stimulation of an immune response against tumors in a subject in need thereof. More specifically the invention provides immunogenic compositions containing bacterial outer membrane vesicles loaded with tumor antigens, fusion proteins comprising a bacterial protein and a tumor antigen, and isolated bacterial outer membrane vesicles (OMVs) containing said fusion proteins. The fusion proteins, OMVs and immunogenic compositions according to the invention are used in the prevention and treatment of tumors.
Claims10
An isolated bacterial outer membrane vesicle comprising a fusion protein, wherein the fusion protein comprises an isolated bacterial protein fused to one or more copies of an immunogenic tumor antigen protein, wherein the isolated bacterial protein is selected from the group consisting of Neisseria meningitidis factor H binding protein (fHbp) of SEQ ID NO:
- 1, Neisseria meningitidis NHBA of SEQ ID NO:
- 109, Escherichia coli outer membrane protein-F (OmpF) of SEQ ID NO:
- 3, and Aggregatibacter actinomycetemcomitans factor H binding protein (Aa-fHbp) of SEQ ID NO:
- 110.
An immunogenic composition comprising a mixture of isolated bacterial outer membrane vesicles each comprising a fusion protein, wherein the fusion protein comprises an isolated bacterial protein fused to an immunogenic tumor antigen protein, wherein the isolated bacterial protein is selected from the group consisting of Neisseria meningitidis factor H binding protein (fHbp) of SEQ ID NO:
- 1, Neisseria meningitidis NHBA of SEQ ID NO:
- 109, Escherichia coli outer membrane protein-F (OmpF) of SEQ ID NO:
- 3, and Aggregatibacter actinomycetemcomitans factor H binding protein (Aa-fHbp) of SEQ ID NO:
- 110 and wherein each of the bacterial outer membrane vesicle differs from the other in the type of the immunogenic tumor antigen protein comprised in the fusion protein.
Dependencies are inferred from the claim text.