Assay modules having assay reagents and methods of making and using same

A multi-well assay plate comprising a plate body with a plurality of wells defined therein comprising:

• (a) a plurality of detection wells, wherein said detection wells
• (i) having well floors and well walls, wherein said well walls have inner wall surfaces and outer wall surfaces,
• (ii) are arranged in a regular two dimensional pattern, and
• (iii) comprise, on an inner surface of each of said detection wells, a binding surface having capture reagent immobilized thereon an array;
• and
• (b) a plurality of reagent reconstitution wells, wherein said reagent reconstitution wells
• (i) have well floors and well walls, wherein said well walls are defined by outer wall surfaces of said detection wells and by rib elements connecting the outer wall surface of adjacent detection wells and
• (ii) comprise, in each reagent reconstitution well, a reconstitutable dry labeled detection reagent;
• wherein at least one detection well and one reagent reconstitution well comprise matched capture and detection reagents for measuring an analyte of interest, and wherein said capture reagent and said reconstitutable dry labeled detection reagent are not in the same well.