Method of producing glycine by fermentation
AssigneeAjinomoto Co., Inc.
InventorsSergey Vasilievich Smirnov, Veronika Aleksandrovna Kotliarova
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Abstract
The present invention provides a method for producing glycine or a salt thereof by fermentation of a bacterium which has been modified to overexpress a gene encoding a protein having L-threonine 3-dehydrogenase activity and a gene encoding a protein having 2-amino-3-oxobutanoate coenzyme A ligase activity. The bacterium can be, for example, a coryneform bacterium or a bacterium belonging to the family Enterobacteriaceae.
Claims7
A method for producing glycine or a salt thereof comprising:
- (i) cultivating a glycine-producing bacterium in a culture medium to produce and accumulate the glycine or a salt thereof in the culture medium, and
- (ii) collecting the glycine or the salt thereof from the culture medium;
- wherein the bacterium has been modified to overexpress a gene encoding a protein having L-threonine 3-dehydrogenase activity and a gene encoding a protein having 2-amino-3-oxobutanoate coenzyme A ligase activity,
- wherein said bacterium belongs to the genus Corynebacterium, and
- wherein said protein having L-threonine 3-dehydrogenase activity is selected from the group consisting of:
- (A) a protein comprising the amino acid sequence shown in SEQ ID NO:
- 2,
- (B) a protein comprising the amino acid sequence shown in SEQ ID NO:
- 2, but which includes substitution, deletion, insertion, and/or addition of 1 to 50 amino acid residues, and wherein said protein has L-threonine 3-dehydrogenase activity,
- (C) a protein having an identity of amino acid residues of not less than 90% with respect to the entire amino acid sequence shown in SEQ ID NO:
- 2, and wherein said protein has L-threonine 3-dehydrogenase activity;
- and wherein said protein having 2-amino-3-oxobutanoate coenzyme A ligase activity is selected from the group consisting of:
- (D) a protein comprising the amino acid sequence shown in SEQ ID NO:
- 4,
- (E) a protein comprising the amino acid sequence shown in SEQ ID NO:
- 4, but which includes substitution, deletion, insertion, and/or addition of 1 to 50 amino acid residues, and wherein said protein has 2-amino-3-oxobutanoate coenzyme A ligase activity,
- (F) a protein having an identity of amino acid residues of not less than 90% with respect to the entire amino acid sequence shown in SEQ ID NO:
- 4, and wherein said protein has 2-amino-3-oxobutanoate coenzyme A ligase activity.
A glycine-producing bacterium, wherein said bacterium has been modified to overexpress a gene encoding a protein having L-threonine 3-dehydrogenase activity and a gene encoding a protein having 2-amino-3-oxobutanoate coenzyme A ligase activity,
- wherein said bacterium belongs to the genus Corynebacterium, and
- wherein said protein having L-threonine 3-dehydrogenase activity is selected from the group consisting of:
- (A) a protein comprising the amino acid sequence shown in SEQ ID NO:
- 2,
- (B) a protein comprising the amino acid sequence shown in SEQ ID NO:
- 2, but which includes substitution, deletion, insertion, and/or addition of 1 to 50 amino acid residues, and wherein said protein has L-threonine 3-dehydrogenase activity,
- (C) a protein having an identity of amino acid residues of not less than 90% with respect to the entire amino acid sequence shown in SEQ ID NO:
- 2, and wherein said protein has L-threonine 3-dehydrogenase activity;
- and wherein said protein having 2-amino-3-oxobutanoate coenzyme A ligase activity is selected from the group consisting of:
- (D) a protein comprising the amino acid sequence shown in SEQ ID NO:
- 4,
- (E) a protein comprising the amino acid sequence shown in SEQ ID NO:
- 4, but which includes substitution, deletion, insertion, and/or addition of 1 to 50 amino acid residues, and wherein said protein has 2-amino-3-oxobutanoate coenzyme A ligase activity,
- (F) a protein having an identity of amino acid residues of not less than 90% with respect to the entire amino acid sequence shown in SEQ ID NO:
- 4, and wherein said protein has 2-amino-3-oxobutanoate coenzyme A ligase activity.
Dependencies are inferred from the claim text.