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AssigneeGenomatica, Inc.
InventorsDerek L. Greenfield, Andreas W. Schirmer, Elizabeth J. Clarke, Eli S. Groban, Bernardo M. Da Costa, Zhihao Hu, Kevin Holden, Noah Helman
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The invention relates to compositions and methods, including polynucleotide sequences, amino acid sequences, recombinant host cells and recombinant host cell cultures engineered to produce fatty acid derivative compositions comprising fatty acids, fatty alcohols, fatty aldehydes, fatty esters, alkanes, terminal olefins, internal olefins or ketones. The fatty acid derivative composition is produced extracellularly with a higher titer, yield or productivity than the corresponding wild type or non-engineered host cell.
A recombinant host cell comprising a transposon insertion in a yijP gene, wherein the yijP gene is flanked by a gene encoding a phosphoenolpyruvate carboxylase (ppc) polypeptide, and wherein the transposon insertion down-regulates the ppc gene, and wherein the recombinant host cell further expresses an exogenous polynucleotide encoding a thioesterase, wherein said recombinant host cell produces a fatty acid derivative composition at a higher titer, yield or productivity than a corresponding wild type host cell when cultured in a medium containing a carbon source under conditions effective to express said ppc polypeptide.
Dependencies are inferred from the claim text.