Methods and microorganisms for producing flavors and fragrance chemicals
AssigneeArdra Inc.
InventorsPratish Gawand, Jonas Ernst Norbert Muller
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Abstract
The present disclosure relates to biosynthetic pathways for producing flavor and fragrance chemicals, such as green notes including trans-2-unsaturated aldehydes and lactones. The present disclosure provides methods for producing trans-2-unsaturated aldehydes, delta-lactones, and gamma-lactones. The present disclosure provides pathways for the preparation of trans-2-unsaturated aldehydes, delta-lactones, and gamma-lactones by reacting aldehydes in the presence of aldolases.
Claims10
A non-naturally occurring microorganism having a trans-2-unsaturated aldehyde pathway, wherein the non-naturally occurring microorganism is transformed with an exogenous nucleic acid encoding an aldolase comprising the amino acid sequence of SEQ ID NOs:
- 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20, an exogenous nucleic acid encoding a decarboxylase comprising the amino acid sequence of SEQ ID NOs:
- 68, 71, 76, or 77 and an exogenous nucleic acid encoding an aldo-ketoreductase comprising the amino acid sequence of SEQ ID NOs:
- 30, 31, 32, or 33, wherein the non-naturally occurring microorganism produces a trans-2-unsaturated aldehyde.
Dependencies are inferred from the claim text.