Vaccines against infectious diseases caused by positive stranded RNA viruses
AssigneeMedigen, Inc.
InventorsPeter Pushko, Irina Tretyakova
Life of this patent
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Abstract
Compositions for protecting subjects from diseases caused by (+) SS RNA virus are described herein. The compositions include (i) a vector containing a DNA encoding a RNA molecule of an infectious (+) SS RNA virus operably linked to a eukaryotic RNA polymerase promoter and a carrier; or (ii) (+) SS RNA viruses obtained from eukaryotic cells transfected with the vector of (i) and a carrier.
Claims27
A vector comprising a DNA encoding a RNA molecule operably linked to a promoter suitable for expression of the DNA in a eukaryotic cell, wherein the RNA molecule encodes an infectious positive single stranded ((+)SS) RNA virus, wherein the (+)SS RNA virus is from the family Flaviviridae, wherein the DNA comprises three introns, and wherein
- the DNA encoding the RNA molecule comprises SEQ ID NO:
- 1;
- the DNA encoding the RNA molecule comprises at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% sequence identity with SEQ ID NO:
- 1, and the introns in SEQ ID NO:
- 1 are intact;
- the DNA comprises at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% sequence identity with a DNA encoding nucleic acid sequence of JEV SA14-14-2 strain and the introns are inserted immediately after nucleotide 414, nucleotide 2213, and nucleotide 3134 of the DNA encoding the nucleic acid sequence of JEV SA14-14-2 strain;
- or
- the DNA encodes nucleic acid sequence of JEV SA14-14-2 strain and the introns are inserted immediately after nucleotide 414, nucleotide 2213, and nucleotide 3134 of the DNA encoding the nucleic acid sequence of JEV SA14-14-2 strain.
A vector comprising a DNA encoding a chimeric RNA molecule of a chimeric infectious (+)SS RNA virus, wherein the DNA is operably linked to a promoter suitable for expression of the DNA in a eukaryotic cell, wherein the chimeric RNA molecule comprises nucleic acid sequences from at least two different (+)SS RNA viruses from the family Flaviviridae, wherein the DNA comprises three introns, and wherein
- the DNA encoding the chimeric RNA molecule comprises nucleotides 1001 to 4477 of SEQ ID NO:
- 1;
- and
- the DNA encoding the chimeric RNA comprises at least 80% or 85% of the entire nucleic acid sequence of JEV SA14-14-2 strain.
A method of preparing a stable plasmid, wherein the method comprises a DNA encoding a genomic RNA of an infectious (+)SS RNA virus, wherein the (+)SS RNA virus is from the family Flaviviridae, wherein the method comprises introducing three introns into the DNA, and wherein
- the DNA encoding the RNA molecule comprises SEQ ID NO:
- 1;
- the DNA encoding the RNA molecule comprises at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% sequence identity with SEQ ID NO:
- 1, and the introns in SEQ ID NO:
- 1 are intact;
- the DNA comprises at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% sequence identity with a DNA encoding nucleic acid sequence of JEV SA14-14-2 strain and the introns are inserted immediately after nucleotide 414, nucleotide 2213, and nucleotide 3134 of the DNA encoding the nucleic acid sequence of JEV SA14-14-2 strain;
- or
- the DNA encodes nucleic acid sequence of JEV SA14-14-2 strain and the introns are inserted immediately after nucleotide 414, nucleotide 2213, and nucleotide 3134 of the DNA encoding the nucleic acid sequence of JEV SA14-14-2 strain.
Dependencies are inferred from the claim text.