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US 11,053,528 B2Granted
Enzymatic production of D-allulose
AssigneeBonumose, Inc.
InventorsDaniel Joseph Wichelecki, Edwin O. Rogers
Life of this patent
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Abstract
The current disclosure provides a process for enzymatically converting a saccharide into allulose. The invention also relates to a process for preparing allulose where the process involves converting fructose 6-phosphate (F6P) to allulose 6-phosphate (A6P), catalyzed by allulose 6-phosphate 3-epimerase (A6PE), and converting the A6P to allulose, catalyzed by allulose 6-phosphate phosphatase (A6PP).
Claims7
An enzymatic process for preparing D-allulose from a starch derivative, the process comprising the steps of:
- (i) converting the starch derivative to glucose 1-phosphate (G1P), catalyzed by an alpha glucan phosphorylase (αGP);
- (ii) converting the G1P to glucose 6-phosphate (G6P), catalyzed by a phosphoglucomutase (PGM);
- (iii) converting the G6P to fructose 6-phosphate (F6P), catalyzed by a phosphoglucoisomerase (PGI);
- (iv) converting the F6P to allulose 6-phosphate (A6P), catalyzed by an allulose 6-phosphate epimerase (A6PE);
- and
- (v) converting the A6P to D-allulose, catalyzed by an allulose 6-phosphate phosphatase (A6PP), wherein the A6PP dephosphorylate the A6P to produce the D-allulose and phosphate ions.
3 more elements
Dependencies are inferred from the claim text.