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US 11,053,519 B2Granted
Method for producing objective substance
AssigneeAjinomoto Co., Inc.
InventorsBenjamin Mijts, Christine Roche, Peter Kelly, Sayaka Asari, Keita Fukui, Miku Toyazaki
Life of this patent
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Abstract
A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism having an objective substance-producing ability, which microorganism has been modified so that the activity of S-adenosyl-L-homocysteine hydrolase is increased.
Claims23
A method for producing an objective substance, the method comprising the following step:
- producing the objective substance by using a microorganism having an ability to produce the objective substance,
- wherein the microorganism has been modified so that the activity of S-adenosyl-L-homocysteine hydrolase is increased as compared with a non-modified microorganism,
- wherein the microorganism is a coryneform bacterium or yeast,
- wherein the objective substance is selected from the group consisting of vanillin, vanillic acid, ergothioneine, mugineic acid, ferulic acid, polyamine, guaiacol, 4-vinylguaiacol, 4-ethylguaiacol, and creatine, and
- wherein said producing comprises:
- cultivating the microorganism in a culture medium containing a carbon source to produce and accumulate the objective substance in the culture medium, or
- converting a precursor of the objective substance into the objective substance by:
- cultivating the microorganism in a culture medium containing the precursor to produce and accumulate the objective substance in the culture medium, or
- allowing cells of the microorganism to act on the precursor in a reaction mixture to produce and accumulate the objective substance in the reaction mixture.
6 more elements
Dependencies are inferred from the claim text.