Multiwell cell culture system having rotating shafts for mixing culture media and method of use thereof

A method of producing organoids comprising:

• a) providing a cell culture system, the cell culture system comprising:
• a first multiwell culture plate, the first multiwell culture plate having:
• i) a base substrate having a plurality of culture wells;
• and
• ii) a shaft operably associated with each culture well, each shaft being configured to mix media present in each culture well and having a gear adapted to operably associate with a gear on a shaft associated with an adjacent culture well;
• a second multiwell culture plate, the second multiwell culture plate having:
• i) a base substrate having a plurality of culture wells;
• and
• ii) a shaft associated with each culture well, each shaft being configured to mix media present in each culture well and having a gear adapted to operably associate with a gear on a shaft associated with an adjacent culture well;
• and
• a motor having a drive shaft in operable communication with the shaft gears of the first and second multiwell culture plates,
• wherein rotation of the drive shaft causes rotation of each shaft of the first and second multiwell culture plates and mixing of the media in each culture well of the first and second multiwell culture plates, and
• wherein the first and second multiwell culture plates are in a stacked configuration;
• b) culturing a cell in a culture well of the first or second multiwell culture plate in culture media under conditions suitable for cell culture, wherein the conditions comprise mixing of the cell culture via actuation of the motor;
• and
• c) optionally harvesting organoids from the culture well;
• thereby producing organoids.