Back to results
US 11,051,532 B2Granted
Methods for purifying protein
AssigneeImpossible Foods Inc.
InventorsCarl Allen Henderson, Jr., Ratnayake Mudiyanselage Dunilka Nishani Ratnayake, Ian Guiles Ronningen, Laila Dafik
Life of this patent
Estimating the term…
Abstract
This disclosure describes methods for purifying protein, and more particularly to methods for purifying protein that minimize the development of undesirable odors and flavors in the purified protein and increase protein yield.
Claims13
A method for purifying protein from a plurality of cells, the method comprising:
- a) lysing an aqueous suspension of the plurality of cells to obtain a cell lysate;
- b) clarifying the cell lysate, optionally in the presence of one or more flocculants, to obtain a clarified lysate;
- c) filtering the clarified lysate to obtain a protein composition;
- and
- d) optionally pasteurizing the protein composition, to obtain a pasteurized protein composition,
- wherein steps a), b), c), and d) independently, are performed at a pH between about 8.5 and about 12.0.
3 more elements
A method for purifying protein from a plurality of cells, the method comprising:
- a) lysing an aqueous suspension of the plurality of cells to obtain a cell lysate;
- b) filtering the cell lysate to obtain a protein composition;
- and
- c) optionally pasteurizing the protein composition to obtain a pasteurized protein composition,
- wherein steps a), b), and c) independently, are performed at a pH between about 8.5 and about 12.0.
2 more elements
Dependencies are inferred from the claim text.